4WHJ

Myxovirus Resistance Protein 2 (MxB)

4WHJ の概要

• エントリーDOI: 10.2210/pdb4whj/pdb
• 分子名称: Interferon-induced GTP-binding protein Mx2 (1 entity in total)
• 機能のキーワード: dimer, 4-helix bundle, gtpase, antiviral protein, hydrolase
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cytoplasm : P20592
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 147777.56

構造登録者

Xiong, Y.,Fribourgh, J.L.,Nguyen, H.C. (登録日: 2014-09-22, 公開日: 2014-10-29, 最終更新日: 2023-09-27)

主引用文献

Fribourgh, J.L.,Nguyen, H.C.,Matreyek, K.A.,Alvarez, F.J.,Summers, B.J.,Dewdney, T.G.,Aiken, C.,Zhang, P.,Engelman, A.,Xiong, Y.
Structural Insight into HIV-1 Restriction by MxB.
Cell Host Microbe, 16:627-638, 2014

PubMed Abstract: The myxovirus resistance (Mx) proteins are interferon-induced dynamin GTPases that can inhibit a variety of viruses. Recently, MxB, but not MxA, was shown to restrict HIV-1 by an unknown mechanism that likely occurs in close proximity to the host cell nucleus and involves the viral capsid. Here, we present the crystal structure of MxB and reveal determinants involved in HIV-1 restriction. MxB adopts an extended antiparallel dimer and dimerization, but not higher-ordered oligomerization, is critical for restriction. Although MxB is structurally similar to MxA, the orientation of individual domains differs between MxA and MxB, and their antiviral functions rely on separate determinants, indicating distinct mechanisms for virus inhibition. Additionally, MxB directly binds the HIV-1 capsid, and this interaction depends on dimerization and the N terminus of MxB as well as the assembled capsid lattice. These insights establish a framework for understanding the mechanism by which MxB restricts HIV-1.

PubMed: 25312384
DOI: 10.1016/j.chom.2014.09.021

実験手法

X-RAY DIFFRACTION (3.2 Å)