4WCW

Ribosomal silencing factor during starvation or stationary phase (RsfS) from Mycobacterium tuberculosis

4WCW の概要

• エントリーDOI: 10.2210/pdb4wcw/pdb
• 分子名称: Ribosomal silencing factor RsfS, MAGNESIUM ION, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (4 entities in total)
• 機能のキーワード: tuberculosis ribosomal silencing dimer, structural genomics, tb structural genomics consortium, tbsgc, translation
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 61490.64

構造登録者

Li, X.,Sun, Q.,Jiang, C.,Yang, K.,Hung, L.,Zhang, J.,Sacchettini, J.,TB Structural Genomics Consortium (TBSGC) (登録日: 2014-09-05, 公開日: 2014-09-24, 最終更新日: 2023-09-27)

主引用文献

Li, X.,Sun, Q.,Jiang, C.,Yang, K.,Hung, L.W.,Zhang, J.,Sacchettini, J.C.
Structure of Ribosomal Silencing Factor Bound to Mycobacterium tuberculosis Ribosome.
Structure, 23:1858-1865, 2015

PubMed Abstract: The ribosomal silencing factor RsfS slows cell growth by inhibiting protein synthesis during periods of diminished nutrient availability. The crystal structure of Mycobacterium tuberculosis (Mtb) RsfS, together with the cryo-electron microscopy (EM) structure of the large subunit 50S of Mtb ribosome, reveals how inhibition of protein synthesis by RsfS occurs. RsfS binds to the 50S at L14, which, when occupied, blocks the association of the small subunit 30S. Although Mtb RsfS is a dimer in solution, only a single subunit binds to 50S. The overlap between the dimer interface and the L14 binding interface confirms that the RsfS dimer must first dissociate to a monomer in order to bind to L14. RsfS interacts primarily through electrostatic and hydrogen bonding to L14. The EM structure shows extended rRNA density that it is not found in the Escherichia coli ribosome, the most striking of these being the extended RNA helix of H54a.

PubMed: 26299947
DOI: 10.1016/j.str.2015.07.014

実験手法

X-RAY DIFFRACTION (2.1 Å)