4WBQ

Crystal structure of the exonuclease domain of QIP (QDE-2 interacting protein) solved by native-SAD phasing.

Summary for 4WBQ

• Entry DOI: 10.2210/pdb4wbq/pdb
• Descriptor: QDE-2-interacting protein, CALCIUM ION (3 entities in total)
• Functional Keywords: rna binding protein
• Biological source: Neurospora crassa
• Total number of polymer chains: 2
• Total formula weight: 53750.19

Authors

Boland, A.,Weinert, T.,Weichenrieder, O.,Wang, M. (deposition date: 2014-09-03, release date: 2014-12-10, Last modification date: 2024-05-08)

Primary citation

Weinert, T.,Olieric, V.,Waltersperger, S.,Panepucci, E.,Chen, L.,Zhang, H.,Zhou, D.,Rose, J.,Ebihara, A.,Kuramitsu, S.,Li, D.,Howe, N.,Schnapp, G.,Pautsch, A.,Bargsten, K.,Prota, A.E.,Surana, P.,Kottur, J.,Nair, D.T.,Basilico, F.,Cecatiello, V.,Pasqualato, S.,Boland, A.,Weichenrieder, O.,Wang, B.C.,Steinmetz, M.O.,Caffrey, M.,Wang, M.
Fast native-SAD phasing for routine macromolecular structure determination.
Nat.Methods, 12:131-133, 2015

PubMed Abstract: We describe a data collection method that uses a single crystal to solve X-ray structures by native SAD (single-wavelength anomalous diffraction). We solved the structures of 11 real-life examples, including a human membrane protein, a protein-DNA complex and a 266-kDa multiprotein-ligand complex, using this method. The data collection strategy is suitable for routine structure determination and can be implemented at most macromolecular crystallography synchrotron beamlines.

PubMed: 25506719
DOI: 10.1038/nmeth.3211

Experimental method

X-RAY DIFFRACTION (2.693 Å)