4V9D

Structures of the bacterial ribosome in classical and hybrid states of tRNA binding

これはPDB形式変換不可エントリーです。

「3R8N」から置き換えられました 「3R8O」から置き換えられました

4V9D の概要

• エントリーDOI: 10.2210/pdb4v9d/pdb
• 分子名称: 16S rRNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (60 entities in total)
• 機能のキーワード: protein biosynthesis, rna, trna, transfer rna, 30s, 70s, 16s, ribosomal subunit, ribosome recycling factor, rrf, ribosome
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cytoplasm: P0A805
• タンパク質・核酸の鎖数: 109
• 化学式量合計: 4322347.90

構造登録者

Dunkle, J.A.,Wang, L.,Feldman, M.B.,Pulk, A.,Chen, V.B.,Kapral, G.J.,Noeske, J.,Richardson, J.S.,Blanchard, S.C.,Cate, J.H.D. (登録日: 2012-07-31, 公開日: 2014-07-09, 最終更新日: 2023-09-20)

主引用文献

Dunkle, J.A.,Wang, L.,Feldman, M.B.,Pulk, A.,Chen, V.B.,Kapral, G.J.,Noeske, J.,Richardson, J.S.,Blanchard, S.C.,Cate, J.H.
Structures of the bacterial ribosome in classical and hybrid states of tRNA binding.
Science, 332:981-984, 2011

PubMed Abstract: During protein synthesis, the ribosome controls the movement of tRNA and mRNA by means of large-scale structural rearrangements. We describe structures of the intact bacterial ribosome from Escherichia coli that reveal how the ribosome binds tRNA in two functionally distinct states, determined to a resolution of ~3.2 angstroms by means of x-ray crystallography. One state positions tRNA in the peptidyl-tRNA binding site. The second, a fully rotated state, is stabilized by ribosome recycling factor and binds tRNA in a highly bent conformation in a hybrid peptidyl/exit site. The structures help to explain how the ratchet-like motion of the two ribosomal subunits contributes to the mechanisms of translocation, termination, and ribosome recycling.

PubMed: 21596992
DOI: 10.1126/science.1202692

実験手法

X-RAY DIFFRACTION (3 Å)