4V91

Kluyveromyces lactis 80S ribosome in complex with CrPV-IRES

これはPDB形式変換不可エントリーです。

4V91 の概要

• エントリーDOI: 10.2210/pdb4v91/pdb
• 関連するPDBエントリー: 4CUX 4CUY
• EMDBエントリー: 2599
• 分子名称: 25S RRNA, EL8, UL6, ... (45 entities in total)
• 機能のキーワード: translation, initiation, ribosome, ires
• 由来する生物種: KLUYVEROMYCES LACTIS; 詳細
• タンパク質・核酸の鎖数: 45
• 化学式量合計: 1977859.37

構造登録者

Fernandez, I.S.,Bai, X.,Scheres, S.H.W.,Ramakrishnan, V. (登録日: 2014-03-21, 公開日: 2014-07-09, 最終更新日: 2024-11-20)

主引用文献

Fernandez, I.S.,Bai, X.,Murshudov, G.,Scheres, S.H.W.,Ramakrishnan, V.
Initiation of Translation by Cricket Paralysis Virus Ires Requires its Translocation in the Ribosome.
Cell(Cambridge,Mass.), 157:823-, 2014

PubMed Abstract: The cricket paralysis virus internal ribosome entry site (CrPV-IRES) is a folded structure in a viral mRNA that allows initiation of translation in the absence of any host initiation factors. By using recent advances in single-particle electron cryomicroscopy, we have solved the structure of CrPV-IRES bound to the ribosome of the yeast Kluyveromyces lactis in both the canonical and rotated states at overall resolutions of 3.7 and 3.8 Å, respectively. In both states, the pseudoknot PKI of the CrPV-IRES mimics a tRNA/mRNA interaction in the decoding center of the A site of the 40S ribosomal subunit. The structure and accompanying factor-binding data show that CrPV-IRES binding mimics a pretranslocation rather than initiation state of the ribosome. Translocation of the IRES by elongation factor 2 (eEF2) is required to bring the first codon of the mRNA into the A site and to allow the start of translation.

PubMed: 24792965
DOI: 10.1016/J.CELL.2014.04.015

実験手法

ELECTRON MICROSCOPY (3.7 Å)