4V8Y

Cryo-EM reconstruction of the 80S-eIF5B-Met-itRNAMet Eukaryotic Translation Initiation Complex

これはPDB形式変換不可エントリーです。

4V8Y の概要

• エントリーDOI: 10.2210/pdb4v8y/pdb
• 関連するPDBエントリー: 4BYT 4BYU 4BYV 4BYW 4BYX
• EMDBエントリー: 2421
• 分子名称: 40S RIBOSOMAL PROTEIN S26-A, 40S RIBOSOMAL PROTEIN S1-A, 40S RIBOSOMAL PROTEIN S2, ... (90 entities in total)
• 機能のキーワード: ribosome, ribosome initiation complex, initiator factor eif5b, single particle analysis
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); 詳細
• タンパク質・核酸の鎖数: 87
• 化学式量合計: 3376539.30

構造登録者

Fernandez, I.S.,Bai, X.C.,Hussain, T.,Kelley, A.C.,Lorsch, J.R.,Ramakrishnan, V.,Scheres, S.H.W. (登録日: 2013-07-20, 公開日: 2014-07-09, 最終更新日: 2024-10-16)

主引用文献

Fernandez, I.S.,Bai, X.C.,Hussain, T.,Kelley, A.C.,Lorsch, J.R.,Ramakrishnan, V.,Scheres, S.H.W.
Molecular architecture of a eukaryotic translational initiation complex.
Science, 342:1240585-1240585, 2013

PubMed Abstract: The last step in eukaryotic translational initiation involves the joining of the large and small subunits of the ribosome, with initiator transfer RNA (Met-tRNA(i)(Met)) positioned over the start codon of messenger RNA in the P site. This step is catalyzed by initiation factor eIF5B. We used recent advances in cryo-electron microscopy (cryo-EM) to determine a structure of the eIF5B initiation complex to 6.6 angstrom resolution from <3% of the population, comprising just 5143 particles. The structure reveals conformational changes in eIF5B, initiator tRNA, and the ribosome that provide insights into the role of eIF5B in translational initiation. The relatively high resolution obtained from such a small fraction of a heterogeneous sample suggests a general approach for characterizing the structure of other dynamic or transient biological complexes.

PubMed: 24200810
DOI: 10.1126/science.1240585

実験手法

ELECTRON MICROSCOPY (4.3 Å)