4V85

Crystal Structure of Release Factor RF3 Trapped in the GTP State on a Rotated Conformation of the Ribosome.

これはPDB形式変換不可エントリーです。

4V85 の概要

• エントリーDOI: 10.2210/pdb4v85/pdb
• 関連するBIRD辞書のPRD_ID: PRD_000226
• 分子名称: 16S rRNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (59 entities in total)
• 機能のキーワード: typeii release factor binding with ribosome, ribosome-antibiotic complex, ribosome/antibiotic
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 59
• 化学式量合計: 2274150.43

構造登録者

Zhou, J.,Lancaster, L.,Trakhanov, S.,Noller, H.F. (登録日: 2011-06-13, 公開日: 2014-07-09, 最終更新日: 2019-07-17)

主引用文献

Zhou, J.,Lancaster, L.,Trakhanov, S.,Noller, H.F.
Crystal structure of release factor RF3 trapped in the GTP state on a rotated conformation of the ribosome.
Rna, 18:230-240, 2012

PubMed Abstract: The class II release factor RF3 is a GTPase related to elongation factor EF-G, which catalyzes release of class I release factors RF1 and RF2 from the ribosome after termination of protein synthesis. The 3.3 Å crystal structure of the RF3·GDPNP·ribosome complex provides a high-resolution description of interactions and structural rearrangements that occur when binding of this translational GTPase induces large-scale rotational movements in the ribosome. RF3 induces a 7° rotation of the body and 14° rotation of the head of the 30S ribosomal subunit, and itself undergoes inter- and intradomain conformational rearrangements. We suggest that ordering of critical elements of switch loop I and the P loop, which help to form the GTPase catalytic site, are caused by interactions between the G domain of RF3 and the sarcin-ricin loop of 23S rRNA. The rotational movements in the ribosome induced by RF3, and its distinctly different binding orientation to the sarcin-ricin loop of 23S rRNA, raise interesting implications for the mechanism of action of EF-G in translocation.

PubMed: 22187675
DOI: 10.1261/rna.031187.111

実験手法

X-RAY DIFFRACTION (3.2 Å)