4V82

Crystal structure of cyanobacterial Photosystem II in complex with terbutryn

This is a non-PDB format compatible entry.

Summary for 4V82

• Entry DOI: 10.2210/pdb4v82/pdb
• Related: 1W5C 2AXT 3BZ1 3BZ2 3KZI 3PRR
• Descriptor: Photosystem Q(B) protein 1, Photosystem II reaction center protein K, Photosystem II reaction center protein L, ... (37 entities in total)
• Functional Keywords: transmembrane alpha-helix, ps ii, ps2, electron transport photosystem, herbicide resistance, photosynthesis, photosystem ii, reaction center, iron, metal-binding, heme, manganese, membrane complex, thylakoid
• Biological source: Thermosynechococcus elongatus; More
• Cellular location: Cellular thylakoid membrane {ECO:0000269|PubMed:14764885, ECO:0000269|PubMed:16355230, ECO:0000269|PubMed:19219048, ECO:0000269|PubMed:20558739, ECO:0000269|PubMed:21367867, ECO:0000269|PubMed:22665786, ECO:0000269|PubMed:23413188, ECO:0000269|PubMed:25006873, ECO:0000269|PubMed:25043005, ECO:0000269|Ref: P0A444 Q8DIQ1 Q8DIF8; Cellular thylakoid membrane ; Single-pass membrane protein : Q9F1K9 Q8DIN8 Q8DHA7 Q8DIQ0 Q8DJI1 Q9F1R6 Q8DJ43 Q8DJZ6 P59087; Cellular thylakoid membrane ; Peripheral membrane protein ; Lumenal side : P0A431 Q9F1L5; Cellular thylakoid membrane {ECO:0000255|HAMAP-Rule:MF_01378, ECO:0000269|PubMed:12881497, ECO:0000269|PubMed:14764885, ECO:0000269|PubMed:16355230, ECO:0000269|PubMed:17935689, ECO:0000269|PubMed:19219048, ECO:0000269|PubMed:20558739, ECO:0000269|PubMed:21367867, ECO:0000269|PubMed:22665786, ECO:0000269|PubMed:23413188, ECO:0000269|PubMed:25006873, ECO:0000269|PubMed:25043005, ECO:0000269|Ref: P0A386; Cellular thylakoid membrane ; Multi- pass membrane protein : Q8DHJ2; Cellular thylakoid membrane {ECO:0000255|HAMAP-Rule:MF_01383, ECO:0000269|PubMed:14764885, ECO:0000269|PubMed:16355230, ECO:0000269|PubMed:19219048, ECO:0000269|PubMed:20558739, ECO:0000269|PubMed:21367867, ECO:0000269|PubMed:22665786, ECO:0000269|PubMed:23413188, ECO:0000269|PubMed:25006873, ECO:0000269|PubMed:25043005, ECO:0000269|Ref: Q8CM25; Cellular thylakoid membrane {ECO:0000255|HAMAP-Rule:MF_00642, ECO:0000269|PubMed:14764885, ECO:0000269|PubMed:16049768, ECO:0000269|PubMed:16172937, ECO:0000269|PubMed:16355230, ECO:0000269|PubMed:17935689, ECO:0000269|PubMed:19219048, ECO:0000269|PubMed:20558739, ECO:0000269|PubMed:21367867, ECO:0000269|PubMed:22665786, ECO:0000269|PubMed:23413188, ECO:0000269|PubMed:25006873, ECO:0000269|PubMed:25043005, ECO:0000269|Ref: Q8DIP0; Cellular thylakoid membrane {ECO:0000269|PubMed:14764885, ECO:0000269|PubMed:16355230, ECO:0000269|PubMed:17935689, ECO:0000269|PubMed:19219048, ECO:0000269|PubMed:20558739, ECO:0000269|PubMed:21367867, ECO:0000269|PubMed:22665786, ECO:0000269|PubMed:23413188, ECO:0000269|PubMed:25006873, ECO:0000269|PubMed:25043005, ECO:0000269|Ref: Q8DIN9
• Total number of polymer chains: 40
• Total formula weight: 742076.47

Authors

Gabdulkhakov, A.,Broser, M.,Guskov, A.,Kern, J.,Glockner, C.,Muh, F.,Saenger, W.,Zouni, A. (deposition date: 2010-11-30, release date: 2014-07-09, Last modification date: 2023-11-08)

Primary citation

Broser, M.,Glockner, C.,Gabdulkhakov, A.,Guskov, A.,Buchta, J.,Kern, J.,Muh, F.,Dau, H.,Saenger, W.,Zouni, A.
Structural basis of cyanobacterial photosystem II Inhibition by the herbicide terbutryn
J.Biol.Chem., 286:15964-15972, 2011

PubMed Abstract: Herbicides that target photosystem II (PSII) compete with the native electron acceptor plastoquinone for binding at the Q(B) site in the D1 subunit and thus block the electron transfer from Q(A) to Q(B). Here, we present the first crystal structure of PSII with a bound herbicide at a resolution of 3.2 Å. The crystallized PSII core complexes were isolated from the thermophilic cyanobacterium Thermosynechococcus elongatus. The used herbicide terbutryn is found to bind via at least two hydrogen bonds to the Q(B) site similar to photosynthetic reaction centers in anoxygenic purple bacteria. Herbicide binding to PSII is also discussed regarding the influence on the redox potential of Q(A), which is known to affect photoinhibition. We further identified a second and novel chloride position close to the water-oxidizing complex and in the vicinity of the chloride ion reported earlier (Guskov, A., Kern, J., Gabdulkhakov, A., Broser, M., Zouni, A., and Saenger, W. (2009) Nat. Struct. Mol. Biol. 16, 334-342). This discovery is discussed in the context of proton transfer to the lumen.

PubMed: 21367867
DOI: 10.1074/jbc.M110.215970

Experimental method

X-RAY DIFFRACTION (3.2 Å)