4V6X

Structure of the human 80S ribosome

This is a non-PDB format compatible entry.

Summary for 4V6X

• Entry DOI: 10.2210/pdb4v6x/pdb
• Related: 3J38 3J39 3J3C 3J3E
• EMDB information: 5591 5592
• Descriptor: Elongation factor 2, 40S ribosomal protein S13, 40S ribosomal protein S11, ... (87 entities in total)
• Functional Keywords: eukarya, eukaryotic, ribosomal, 80s, rna, protein synthesis, mass spectrometry, ribosome
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 89
• Total formula weight: 4051275.23

Authors

Anger, A.M.,Armache, J.-P.,Berninghausen, O.,Habeck, M.,Subklewe, M.,Wilson, D.N.,Beckmann, R. (deposition date: 2013-02-27, release date: 2014-07-09, Last modification date: 2024-11-06)

Primary citation

Anger, A.M.,Armache, J.-P.,Berninghausen, O.,Habeck, M.,Subklewe, M.,Wilson, D.N.,Beckmann, R.
Structures of the human and Drosophila 80S ribosome.
Nature, 497:80-85, 2013

PubMed Abstract: Protein synthesis in all cells is carried out by macromolecular machines called ribosomes. Although the structures of prokaryotic, yeast and protist ribosomes have been determined, the more complex molecular architecture of metazoan 80S ribosomes has so far remained elusive. Here we present structures of Drosophila melanogaster and Homo sapiens 80S ribosomes in complex with the translation factor eEF2, E-site transfer RNA and Stm1-like proteins, based on high-resolution cryo-electron-microscopy density maps. These structures not only illustrate the co-evolution of metazoan-specific ribosomal RNA with ribosomal proteins but also reveal the presence of two additional structural layers in metazoan ribosomes, a well-ordered inner layer covered by a flexible RNA outer layer. The human and Drosophila ribosome structures will provide the basis for more detailed structural, biochemical and genetic experiments.

PubMed: 23636399
DOI: 10.1038/nature12104

Experimental method

ELECTRON MICROSCOPY (5 Å)