4V6W

Structure of the D. melanogaster 80S ribosome

これはPDB形式変換不可エントリーです。

4V6W の概要

• エントリーDOI: 10.2210/pdb4v6w/pdb
• 関連するPDBエントリー: 3J3A 3J3B 3J3D 3J3F
• EMDBエントリー: 5591 5592
• 分子名称: Elongation factor 2, 40S ribosomal protein S13, 40S ribosomal protein S11, ... (86 entities in total)
• 機能のキーワード: eukarya, eukaryotic, ribosomal, 80s, rna, protein synthesis, mass spectrometry, ribosome
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 86
• 化学式量合計: 3670249.99

構造登録者

Anger, A.M.,Armache, J.-P.,Berninghausen, O.,Habeck, M.,Subklewe, M.,Wilson, D.N.,Beckmann, R. (登録日: 2013-02-27, 公開日: 2014-07-09, 最終更新日: 2024-05-15)

主引用文献

Anger, A.M.,Armache, J.-P.,Berninghausen, O.,Habeck, M.,Subklewe, M.,Wilson, D.N.,Beckmann, R.
Structures of the human and Drosophila 80S ribosome.
Nature, 497:80-85, 2013

PubMed Abstract: Protein synthesis in all cells is carried out by macromolecular machines called ribosomes. Although the structures of prokaryotic, yeast and protist ribosomes have been determined, the more complex molecular architecture of metazoan 80S ribosomes has so far remained elusive. Here we present structures of Drosophila melanogaster and Homo sapiens 80S ribosomes in complex with the translation factor eEF2, E-site transfer RNA and Stm1-like proteins, based on high-resolution cryo-electron-microscopy density maps. These structures not only illustrate the co-evolution of metazoan-specific ribosomal RNA with ribosomal proteins but also reveal the presence of two additional structural layers in metazoan ribosomes, a well-ordered inner layer covered by a flexible RNA outer layer. The human and Drosophila ribosome structures will provide the basis for more detailed structural, biochemical and genetic experiments.

PubMed: 23636399
DOI: 10.1038/nature12104

実験手法

ELECTRON MICROSCOPY (6 Å)