4V6F

Elongation complex of the 70S ribosome with three tRNAs and mRNA.

これはPDB形式変換不可エントリーです。

4V6F の概要

• エントリーDOI: 10.2210/pdb4v6f/pdb
• 関連するPDBエントリー: 3I8G 3I8H 3I8I
• 分子名称: 23S RIBOSOMAL RNA, 50S RIBOSOMAL PROTEIN L14, 50S RIBOSOMAL PROTEIN L15, ... (61 entities in total)
• 機能のキーワード: ribosome, trna, mrna
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 114
• 化学式量合計: 4678968.83

構造登録者

Jenner, L.B.,Yusupova, G.,Yusupov, M. (登録日: 2009-07-09, 公開日: 2014-07-09, 最終更新日: 2024-11-06)

主引用文献

Jenner, L.B.,Demeshkina, N.,Yusupova, G.,Yusupov, M.
Structural aspects of messenger RNA reading frame maintenance by the ribosome.
Nat.Struct.Mol.Biol., 17:555-560, 2010

PubMed Abstract: One key question in protein biosynthesis is how the ribosome couples mRNA and tRNA movements to prevent disruption of weak codon-anticodon interactions and loss of the translational reading frame during translocation. Here we report the complete path of mRNA on the 70S ribosome at the atomic level (3.1-A resolution), and we show that one of the conformational rearrangements that occurs upon transition from initiation to elongation is a narrowing of the downstream mRNA tunnel. This rearrangement triggers formation of a network of interactions between the mRNA downstream of the A-site codon and the elongating ribosome. Our data elucidate the mechanism by which hypermodified nucleoside 2-methylthio-N6 isopentenyl adenosine at position 37 (ms(2)i(6)A37) in tRNA(Phe)(GAA) stabilizes mRNA-tRNA interactions in all three tRNA binding sites. Another network of contacts is formed between this tRNA modification and ribosomal elements surrounding the mRNA E/P kink, resulting in the anchoring of P-site tRNA. These data allow rationalization of how modification deficiencies of ms(2)i(6)A37 in tRNAs may lead to shifts of the translational reading frame.

PubMed: 20400952
DOI: 10.1038/nsmb.1790

実験手法

X-RAY DIFFRACTION (3.1 Å)