4V6C

Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting

これはPDB形式変換不可エントリーです。

4V6C の概要

• エントリーDOI: 10.2210/pdb4v6c/pdb
• 関連するPDBエントリー: 3I1Q 3I1R 3I1S 3I1T 3I1Z 3I20 3I21 3I22
• 分子名称: 30S ribosomal protein S2, 30S ribosomal protein S11, 30S ribosomal protein S12, ... (57 entities in total)
• 機能のキーワード: ribosome structure, protein-rna complex, ribonucleoprotein, ribosomal protein, rna-binding, rrna-binding, antibiotic resistance, repressor, transcription, transcription regulation, transcription termination, translation regulation, acetylation, trna-binding, methylation, endonuclease, hydrolase, nuclease, ribosome
• 由来する生物種: Escherichia coli K-12; 詳細
• タンパク質・核酸の鎖数: 104
• 化学式量合計: 4294357.38

構造登録者

Zhang, W.,Dunkle, J.A.,Cate, J.H.D. (登録日: 2009-06-27, 公開日: 2014-07-09, 最終更新日: 2024-10-30)

主引用文献

Zhang, W.,Dunkle, J.A.,Cate, J.H.
Structures of the ribosome in intermediate States of ratcheting.
Science, 325:1014-1017, 2009

PubMed Abstract: Protein biosynthesis on the ribosome requires repeated cycles of ratcheting, which couples rotation of the two ribosomal subunits with respect to each other, and swiveling of the head domain of the small subunit. However, the molecular basis for how the two ribosomal subunits rearrange contacts with each other during ratcheting while remaining stably associated is not known. Here, we describe x-ray crystal structures of the intact Escherichia coli ribosome, either in the apo-form (3.5 angstrom resolution) or with one (4.0 angstrom resolution) or two (4.0 angstrom resolution) anticodon stem-loop tRNA mimics bound, that reveal intermediate states of intersubunit rotation. In the structures, the interface between the small and large ribosomal subunits rearranges in discrete steps along the ratcheting pathway. Positioning of the head domain of the small subunit is controlled by interactions with the large subunit and with the tRNA bound in the peptidyl-tRNA site. The intermediates observed here provide insight into how tRNAs move into the hybrid state of binding that precedes the final steps of mRNA and tRNA translocation.

PubMed: 19696352
DOI: 10.1126/science.1175275

実験手法

X-RAY DIFFRACTION (3.19 Å)