4V49

Crystal Structure of a Streptomycin Dependent Ribosome from E. Coli 70S Ribosome.

これはPDB形式変換不可エントリーです。

4V49 の概要

• エントリーDOI: 10.2210/pdb4v49/pdb
• 関連するPDBエントリー: 1PNU 1PNX 1PNY
• 分子名称: 16S RIBOSOMAL RNA, 30S ribosomal protein S8, 30S ribosomal protein S9, ... (55 entities in total)
• 機能のキーワード: 30s ribosomal subunit, protein-rna complex, protein-protein complex, rna-rna complex, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 56
• 化学式量合計: 2193229.47

構造登録者

Vila-Sanjurjo, A.,Ridgeway, W.K.,Seymaner, V.,Zhang, W.,Santoso, S.,Yu, K.,Cate, J.H.D. (登録日: 2003-06-13, 公開日: 2014-07-09, 最終更新日: 2024-11-06)

主引用文献

Vila-Sanjurjo, A.,Ridgeway, W.K.,Seymaner, V.,Zhang, W.,Santoso, S.,Yu, K.,Cate, J.H.D.
X-ray Crystal Structures of the WT and a Hyper-Accurate Ribosome From Escherichia Coli
Proc.Natl.Acad.Sci.USA, 100:8682-8687, 2003

PubMed Abstract: Protein biosynthesis on the ribosome requires accurate reading of the genetic code in mRNA. Two conformational rearrangements in the small ribosomal subunit, a closing of the head and body around the incoming tRNA and an RNA helical switch near the mRNA decoding site, have been proposed to select for complementary base-pairing between mRNA codons and tRNA anticodons. We determined x-ray crystal structures of the WT and a hyper-accurate variant of the Escherichia coli ribosome at resolutions of 10 and 9 A, respectively, revealing that formation of the intact 70S ribosome from its two subunits closes the conformation of the head of the small subunit independent of mRNA decoding. Moreover, no change in the conformation of the switch helix is observed in two steps of tRNA discrimination. These 70S ribosome structures indicate that mRNA decoding is coupled primarily to movement of the small subunit body, consistent with previous proposals, whereas closing of the head and the helical switch may function in other steps of protein synthesis.

PubMed: 12853578
DOI: 10.1073/pnas.1133380100

実験手法

X-RAY DIFFRACTION (8.7 Å)