4V3R

Designed armadillo repeat protein with 5 internal repeats, 2nd generation C-cap and 3rd generation N-cap.

4V3R の概要

• エントリーDOI: 10.2210/pdb4v3r/pdb
• 関連するPDBエントリー: 4V3O 4V3Q
• 分子名称: YIII_M5_AII, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: de novo protein, protein engineering, repeat protein
• 由来する生物種: SYNTHETIC CONSTRUCT
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 60137.66

構造登録者

Reichen, C.,Madhurantakam, C.,Pluckthun, A.,Mittl, P. (登録日: 2014-10-20, 公開日: 2016-01-13, 最終更新日: 2024-01-10)

主引用文献

Reichen, C.,Madhurantakam, C.,Hansen, S.,Grutter, M.G.,Pluckthun, A.,Mittl, P.R.E.
Structures of Designed Armadillo-Repeat Proteins Show Propagation of Inter-Repeat Interface Effects
Acta Crystallogr.,Sect.D, 72:168-, 2016

PubMed Abstract: The armadillo repeat serves as a scaffold for the development of modular peptide-recognition modules. In order to develop such a system, three crystal structures of designed armadillo-repeat proteins with third-generation N-caps (YIII-type), four or five internal repeats (M-type) and second-generation C-caps (AII-type) were determined at 1.8 Å (His-YIIIM4AII), 2.0 Å (His-YIIIM5AII) and 1.95 Å (YIIIM5AII) resolution and compared with those of variants with third-generation C-caps. All constructs are full consensus designs in which the internal repeats have exactly the same sequence, and hence identical conformations of the internal repeats are expected. The N-cap and internal repeats M1 to M3 are indeed extremely similar, but the comparison reveals structural differences in internal repeats M4 and M5 and the C-cap. These differences are caused by long-range effects of the C-cap, contacting molecules in the crystal, and the intrinsic design of the repeat. Unfortunately, the rigid-body movement of the C-terminal part impairs the regular arrangement of internal repeats that forms the putative peptide-binding site. The second-generation C-cap improves the packing of buried residues and thereby the stability of the protein. These considerations are useful for future improvements of an armadillo-repeat-based peptide-recognition system.

PubMed: 26894544
DOI: 10.1107/S2059798315023116

実験手法

X-RAY DIFFRACTION (1.95 Å)