4V2S

Crystal structure of Hfq in complex with the sRNA RydC

4V2S の概要

• エントリーDOI: 10.2210/pdb4v2s/pdb
• 分子名称: RNA-BINDING PROTEIN HFQ, RYDC (3 entities in total)
• 機能のキーワード: rna binding protein-rna complex, natively unstructured protein, protein-rna recognition, rna binding protein/rna
• 由来する生物種: ESCHERICHIA COLI; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 87775.28

構造登録者

Dimastrogiovanni, D.,Frohlich, K.S.,Bruce, H.A.,Bandyra, K.J.,Hohensee, S.,Vogel, J.,Luisi, B.F. (登録日: 2014-10-14, 公開日: 2015-01-14, 最終更新日: 2024-01-10)

主引用文献

Dimastrogiovanni, D.,Frohlich, K.S.,Bandyra, K.J.,Bruce, H.A.,Hohensee, S.,Vogel, J.,Luisi, B.F.
Recognition of the small regulatory RNA RydC by the bacterial Hfq protein.
Elife, 3:-, 2014

PubMed Abstract: Bacterial small RNAs (sRNAs) are key elements of regulatory networks that modulate gene expression. The sRNA RydC of Salmonella sp. and Escherichia coli is an example of this class of riboregulators. Like many other sRNAs, RydC bears a 'seed' region that recognises specific transcripts through base-pairing, and its activities are facilitated by the RNA chaperone Hfq. The crystal structure of RydC in complex with E. coli Hfq at a 3.48 Å resolution illuminates how the protein interacts with and presents the sRNA for target recognition. Consolidating the protein-RNA complex is a host of distributed interactions mediated by the natively unstructured termini of Hfq. Based on the structure and other data, we propose a model for a dynamic effector complex comprising Hfq, small RNA, and the cognate mRNA target.

PubMed: 25551292
DOI: 10.7554/eLife.05375

実験手法

X-RAY DIFFRACTION (3.48 Å)