4V2R

Ironing out their differences: Dissecting the structural determinants of a phenylalanine aminomutase and ammonia lyase

4V2R の概要

• エントリーDOI: 10.2210/pdb4v2r/pdb
• 関連するPDBエントリー: 4V2Q
• 分子名称: PHENYLALANINE AMINOMUTASE (L-BETA-PHENYLALANINE FORMING) (2 entities in total)
• 機能のキーワード: lyase
• 由来する生物種: TAXUS WALLICHIANA VAR. CHINENSIS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 154995.05

構造登録者

Heberling, M.,Masman, M.,Bartsch, S.,Wybenga, G.G.,Dijkstra, B.W.,Marrink, S.,Janssen, D. (登録日: 2014-10-14, 公開日: 2014-12-10, 最終更新日: 2024-10-23)

主引用文献

Heberling, M.M.,Masman, M.F.,Bartsch, S.,Wybenga, G.G.,Dijkstra, B.W.,Marrink, S.J.,Janssen, D.B.
Ironing out their differences: dissecting the structural determinants of a phenylalanine aminomutase and ammonia lyase.
ACS Chem. Biol., 10:989-997, 2015

PubMed Abstract: Deciphering the structural features that functionally separate ammonia lyases from aminomutases is of interest because it may allow for the engineering of more efficient aminomutases for the synthesis of unnatural amino acids (e.g., β-amino acids). However, this has proved to be a major challenge that involves understanding the factors that influence their activity and regioselectivity differences. Herein, we report evidence of a structural determinant that dictates the activity differences between a phenylalanine ammonia lyase (PAL) and aminomutase (PAM). An inner loop region that closes the active sites of both PAM and PAL was mutated within PAM (PAM residues 77-97) in a stepwise approach to study the effects when the equivalent residue(s) found in the PAL loop were introduced into the PAM loop. Almost all of the single loop mutations triggered a lyase phenotype in PAM. Experimental and computational evidence suggest that the induced lyase features result from inner loop mobility enhancements, which are possibly caused by a 310-helix cluster, flanking α-helices, and hydrophobic interactions. These findings pinpoint the inner loop as a structural determinant of the lyase and mutase activities of PAM.

PubMed: 25494407
DOI: 10.1021/cb500794h

実験手法

X-RAY DIFFRACTION (2.2 Å)