4V2E

FLRT3 LRR domain

Summary for 4V2E

• Entry DOI: 10.2210/pdb4v2e/pdb
• Related: 4V2A 4V2B 4V2C 4V2D
• Descriptor: FIBRONECTIN LEUCINE RICH TRANSMEMBRANE PROTEIN 3 (1 entity in total)
• Functional Keywords: signaling protein, leucine-rich repeat, lrr, unc5
• Biological source: MUS MUSCULUS (HOUSE MOUSE)
• Total number of polymer chains: 2
• Total formula weight: 75115.66

Authors

Seiradake, E.,del Toro, D.,Nagel, D.,Cop, F.,Haertl, R.,Ruff, T.,Seyit-Bremer, G.,Harlos, K.,Border, E.C.,Acker-Palmer, A.,Jones, E.Y.,Klein, R. (deposition date: 2014-10-08, release date: 2014-11-05, Last modification date: 2024-10-23)

Primary citation

Seiradake, E.,Del Toro, D.,Nagel, D.,Cop, F.,Hartl, R.,Ruff, T.,Seyit-Bremer, G.,Harlos, K.,Border, E.C.,Acker-Palmer, A.,Jones, E.Y.,Klein, R.
Flrt Structure: Balancing Repulsion and Cell Adhesion in Cortical and Vascular Development.
Neuron, 84:370-, 2014

PubMed Abstract: FLRTs are broadly expressed proteins with the unique property of acting as homophilic cell adhesion molecules and as heterophilic repulsive ligands of Unc5/Netrin receptors. How these functions direct cell behavior and the molecular mechanisms involved remain largely unclear. Here we use X-ray crystallography to reveal the distinct structural bases for FLRT-mediated cell adhesion and repulsion in neurons. We apply this knowledge to elucidate FLRT functions during cortical development. We show that FLRTs regulate both the radial migration of pyramidal neurons, as well as their tangential spread. Mechanistically, radial migration is controlled by repulsive FLRT2-Unc5D interactions, while spatial organization in the tangential axis involves adhesive FLRT-FLRT interactions. Further, we show that the fundamental mechanisms of FLRT adhesion and repulsion are conserved between neurons and vascular endothelial cells. Our results reveal FLRTs as powerful guidance factors with structurally encoded repulsive and adhesive surfaces.

PubMed: 25374360
DOI: 10.1016/J.NEURON.2014.10.008

Experimental method

X-RAY DIFFRACTION (2.5 Å)