4V0H

Human metallo beta lactamase domain containing protein 1 (hMBLAC1)

4V0H の概要

• エントリーDOI: 10.2210/pdb4v0h/pdb
• 分子名称: METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN 1 1, FE (III) ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: hydrolase, glyoxalase ii family, non-heme iron
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 109635.93

構造登録者

Pettinati, I.,McDonough, M.A.,Brem, J.,Schofield, C.J. (登録日: 2014-09-16, 公開日: 2016-01-13, 最終更新日: 2024-05-08)

主引用文献

Pettinati, I.,Grzechnik, P.,Ribeiro de Almeida, C.,Brem, J.,McDonough, M.A.,Dhir, S.,Proudfoot, N.J.,Schofield, C.J.
Biosynthesis of histone messenger RNA employs a specific 3' end endonuclease.
Elife, 7:-, 2018

PubMed Abstract: Replication-dependent (RD) core histone mRNA produced during S-phase is the only known metazoan protein-coding mRNA presenting a 3' stem-loop instead of the otherwise universal polyA tail. A metallo β-lactamase (MBL) fold enzyme, cleavage and polyadenylation specificity factor 73 (CPSF73), is proposed to be the sole endonuclease responsible for 3' end processing of both mRNA classes. We report cellular, genetic, biochemical, substrate selectivity, and crystallographic studies providing evidence that an additional endoribonuclease, MBL domain containing protein 1 (MBLAC1), is selective for 3' processing of RD histone pre-mRNA during the S-phase of the cell cycle. Depletion of MBLAC1 in cells significantly affects cell cycle progression thus identifying MBLAC1 as a new type of S-phase-specific cancer target.

PubMed: 30507380
DOI: 10.7554/eLife.39865

実験手法

X-RAY DIFFRACTION (1.79 Å)