4UZ1

STRUCTURE OF THE WNT DEACYLASE NOTUM - CRYSTAL FORM III - 1.4A

4UZ1 の概要

• エントリーDOI: 10.2210/pdb4uz1/pdb
• 関連するPDBエントリー: 4UYU 4UYW 4UYZ 4UZ5 4UZ6 4UZ7 4UZ9 4UZA 4UZJ 4UZK 4UZL 4UZQ 4WBH
• 分子名称: NOTUM, 2-acetamido-2-deoxy-beta-D-glucopyranose, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: hydrolase, esterase, extracellular, alpha/beta hydrolase
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Secreted : Q6P988
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44172.61

構造登録者

Zebisch, M.,Jones, E.Y. (登録日: 2014-09-04, 公開日: 2015-02-25, 最終更新日: 2024-10-23)

主引用文献

Kakugawa, S.,Langton, P.F.,Zebisch, M.,Howell, S.A.,Chang, T.,Liu, Y.,Feizi, T.,Bineva, G.,O'Reilly, N.,Snijders, A.P.,Jones, E.Y.,Vincent, J.
Notum Deacylates Wnt Proteins to Suppress Signalling Activity.
Nature, 519:187-, 2015

PubMed Abstract: Signalling by Wnt proteins is finely balanced to ensure normal development and tissue homeostasis while avoiding diseases such as cancer. This is achieved in part by Notum, a highly conserved secreted feedback antagonist. Notum has been thought to act as a phospholipase, shedding glypicans and associated Wnt proteins from the cell surface. However, this view fails to explain specificity, as glypicans bind many extracellular ligands. Here we provide genetic evidence in Drosophila that Notum requires glypicans to suppress Wnt signalling, but does not cleave their glycophosphatidylinositol anchor. Structural analyses reveal glycosaminoglycan binding sites on Notum, which probably help Notum to co-localize with Wnt proteins. They also identify, at the active site of human and Drosophila Notum, a large hydrophobic pocket that accommodates palmitoleate. Kinetic and mass spectrometric analyses of human proteins show that Notum is a carboxylesterase that removes an essential palmitoleate moiety from Wnt proteins and thus constitutes the first known extracellular protein deacylase.

PubMed: 25731175
DOI: 10.1038/NATURE14259

実験手法

X-RAY DIFFRACTION (1.4 Å)