4UW2

Crystal structure of Csm1 in T.onnurineus

4UW2 の概要

• エントリーDOI: 10.2210/pdb4uw2/pdb
• 分子名称: CSM1 (2 entities in total)
• 機能のキーワード: immune system, bacterial immunity, crispr, csm interference csm1, hd domain
• 由来する生物種: THERMOCOCCUS ONNURINEUS NA1
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 356150.47

構造登録者

Jung, T.Y.,An, Y.,Park, K.H.,Lee, M.H.,Oh, B.H.,Woo, E.J. (登録日: 2014-08-08, 公開日: 2015-03-25, 最終更新日: 2015-09-23)

主引用文献

Jung, T.,An, Y.,Park, K.,Lee, M.,Oh, B.,Woo, E.
Crystal Structure of the Csm1 Subunit of the Csm Complex and its Single-Stranded DNA-Specific Nuclease Activity.
Structure, 23:782-, 2015

PubMed Abstract: The CRISPR-Cas system is the RNA-guided immune defense mechanism in bacteria and archaea. Csm1 belongs to the Cas10 family, which is the common signature protein of the type III system. Csm1 is the largest subunit of the Csm interference complex in the type III-A subtype, which targets foreign DNA or RNA. Here, we report crystallographic and biochemical analyses of Thermococcus onnurineus Csm1, revealing a five-domain organization and single-stranded DNA (ssDNA)-specific nuclease activity associated with the N-terminal HD domain. This domain folds into permuted secondary structures in comparison with the HD domain of Cas3 and contains all the catalytically important residues. It exhibited both endo- and exonuclease activities in an Ni(2+) or Mn(2+)-dependent manner. The narrow width of the active-site cleft appears to restrict the substrate specificity to ssDNA and thus to prevent Csm1 from cleaving double-stranded chromosomal DNA. These data suggest that Csm1 may function in DNA interference by the Csm effector complex.

PubMed: 25773141
DOI: 10.1016/J.STR.2015.01.021

実験手法

X-RAY DIFFRACTION (2.632 Å)