4UTS

Room temperature crystal structure of the fast switching M159T mutant of fluorescent protein Dronpa

4UTS の概要

• エントリーDOI: 10.2210/pdb4uts/pdb
• 分子名称: FLUORESCENT PROTEIN DRONPA (2 entities in total)
• 機能のキーワード: fluorescent protein, photoswitching, photochromic
• 由来する生物種: ECHINOPHYLLIA SP. SC22
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 98046.58

構造登録者

Kaucikas, M.,Fitzpatrick, A.,Bryan, E.,Struve, A.,Henning, R.,Kosheleva, I.,Srajer, V.,van Thor, J.J. (登録日: 2014-07-22, 公開日: 2015-06-03, 最終更新日: 2024-10-09)

主引用文献

Kaucikas, M.,Fitzpatrick, A.,Bryan, E.,Struve, A.,Henning, R.,Kosheleva, I.,Srajer, V.,Groenhof, G.,Van Thor, J.J.
Room Temperature Crystal Structure of the Fast Switching M159T Mutant of the Fluorescent Protein Dronpa.
Proteins, 83:397-, 2015

PubMed Abstract: The fluorescent protein Dronpa undergoes reversible photoswitching reactions between the bright "on" and dark "off" states via photoisomerization and proton transfer reactions. We report the room temperature crystal structure of the fast switching Met159Thr mutant of Dronpa at 2.0-Å resolution in the bright on state. Structural differences with the wild type include shifted backbone positions of strand β8 containing Thr159 as well as an altered A-C dimer interface involving strands β7, β8, β10, and β11. The Met159Thr mutation increases the cavity volume for the p-hydroxybenzylidene-imidazolinone chromophore as a result of both the side chain difference and the backbone positional differences.

PubMed: 25524427
DOI: 10.1002/PROT.24742

実験手法

X-RAY DIFFRACTION (2.03 Å)