4UTQ

A structural model of the active ribosome-bound membrane protein insertase YidC

4UTQ の概要

• エントリーDOI: 10.2210/pdb4utq/pdb
• EMDBエントリー: 2705
• 分子名称: MEMBRANE PROTEIN INSERTASE YIDC, ATP SYNTHASE SUBUNIT C (2 entities in total)
• 機能のキーワード: protein transport, protein translocation, bioinformatics, md simulation, membrane
• 由来する生物種: ESCHERICHIA COLI; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 69835.57

構造登録者

Wickles, S.,Singharoy, A.,Andreani, J.,Seemayer, S.,Bischoff, L.,Berninghausen, O.,Soeding, J.,Schulten, K.,vanderSluis, E.O.,Beckmann, R. (登録日: 2014-07-22, 公開日: 2014-07-30, 最終更新日: 2024-05-08)

主引用文献

Wickles, S.,Singharoy, A.,Andreani, J.,Seemayer, S.,Bischoff, L.,Berninghausen, O.,Soeding, J.,Schulten, K.,Van Der Sluis, E.O.,Beckmann, R.
A Structural Model of the Active Ribosome-Bound Membrane Protein Insertase Yidc.
Elife, 3:3035-, 2014

PubMed Abstract: The integration of most membrane proteins into the cytoplasmic membrane of bacteria occurs co-translationally. The universally conserved YidC protein mediates this process either individually as a membrane protein insertase, or in concert with the SecY complex. Here, we present a structural model of YidC based on evolutionary co-variation analysis, lipid-versus-protein-exposure and molecular dynamics simulations. The model suggests a distinctive arrangement of the conserved five transmembrane domains and a helical hairpin between transmembrane segment 2 (TM2) and TM3 on the cytoplasmic membrane surface. The model was used for docking into a cryo-electron microscopy reconstruction of a translating YidC-ribosome complex carrying the YidC substrate FOc. This structure reveals how a single copy of YidC interacts with the ribosome at the ribosomal tunnel exit and identifies a site for membrane protein insertion at the YidC protein-lipid interface. Together, these data suggest a mechanism for the co-translational mode of YidC-mediated membrane protein insertion.

PubMed: 25012291
DOI: 10.7554/ELIFE.03035

実験手法

ELECTRON MICROSCOPY (8 Å)