4USK

Unravelling the B. pseudomallei heptokinase WcbL: from Structure to Drug Discovery.

4USK の概要

• エントリーDOI: 10.2210/pdb4usk/pdb
• 関連するPDBエントリー: 4USM
• 分子名称: PUTATIVE SUGAR KINASE, DIMETHYL SULFOXIDE, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: transferase, capsular polysaccharide, heptopyranose
• 由来する生物種: BURKHOLDERIA PSEUDOMALLEI K96243
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 76281.44

構造登録者

Vivoli, M.,Isupov, M.N.,Nicholas, R.,Hill, A.,Scott, A.,Kosma, P.,Prior, J.,Harmer, N.J. (登録日: 2014-07-09, 公開日: 2016-01-13, 最終更新日: 2024-05-08)

主引用文献

Vivoli, M.,Isupov, M.N.,Nicholas, R.,Hill, A.,Scott, A.E.,Kosma, P.,Prior, J.L.,Harmer, N.J.
Unraveling the B.Pseudomallei Heptokinase Wcbl: From Structure to Drug Discovery.
Chem.Biol., 22:1622-, 2015

PubMed Abstract: Gram-negative bacteria utilize heptoses as part of their repertoire of extracellular polysaccharide virulence determinants. Disruption of heptose biosynthesis offers an attractive target for novel antimicrobials. A critical step in the synthesis of heptoses is their 1-O phosphorylation, mediated by kinases such as HldE or WcbL. Here, we present the structure of WcbL from Burkholderia pseudomallei. We report that WcbL operates through a sequential ordered Bi-Bi mechanism, loading the heptose first and then ATP. We show that dimeric WcbL binds ATP anti-cooperatively in the absence of heptose, and cooperatively in its presence. Modeling of WcbL suggests that heptose binding causes an elegant switch in the hydrogen-bonding network, facilitating the binding of a second ATP molecule. Finally, we screened a library of drug-like fragments, identifying hits that potently inhibit WcbL. Our results provide a novel mechanism for control of substrate binding and emphasize WcbL as an attractive anti-microbial target for Gram-negative bacteria.

PubMed: 26687481
DOI: 10.1016/J.CHEMBIOL.2015.10.015

実験手法

X-RAY DIFFRACTION (1.76 Å)