4UOI

Unexpected structure for the N-terminal domain of Hepatitis C virus envelope glycoprotein E1

4UOI の概要

• エントリーDOI: 10.2210/pdb4uoi/pdb
• 分子名称: GENOME POLYPROTEIN, 2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total)
• 機能のキーワード: viral protein
• 由来する生物種: HEPATITIS C VIRUS
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 59912.51

構造登録者

El Omari, K.,Iourin, O.,Kadlec, J.,Harlos, K.,Grimes, J.M.,Stuart, D.I. (登録日: 2014-06-04, 公開日: 2014-08-20, 最終更新日: 2024-11-20)

主引用文献

El Omari, K.,Iourin, O.,Kadlec, J.,Fearn, R.,Hall, D.R.,Harlos, K.,Grimes, J.M.,Stuart, D.I.
Unexpected Structure for the N-Terminal Domain of Hepatitis C Virus Envelope Glycoprotein E1
Acta Crystallogr.,Sect.D, 70:2197-, 2014

PubMed Abstract: Single-wavelength anomalous dispersion of S atoms (S-SAD) is an elegant phasing method to determine crystal structures that does not require heavy-atom incorporation or selenomethionine derivatization. Nevertheless, this technique has been limited by the paucity of the signal at the usual X-ray wavelengths, requiring very accurate measurement of the anomalous differences. Here, the data collection and structure solution of the N-terminal domain of the ectodomain of HCV E1 from crystals that diffracted very weakly is reported. By combining the data from 32 crystals, it was possible to solve the sulfur substructure and calculate initial maps at 7 Å resolution, and after density modication and phase extension using a higher resolution native data set to 3.5 Å resolution model building was achievable.

PubMed: 25084338
DOI: 10.1107/S139900471401339X

実験手法

X-RAY DIFFRACTION (3.49 Å)