4UDE

An oligomerization domain confers pioneer properties to the LEAFY master floral regulator

4UDE の概要

• エントリーDOI: 10.2210/pdb4ude/pdb
• 分子名称: GINLFY PROTEIN, TETRAETHYLENE GLYCOL, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: sam, transcription, oligomerisation
• 由来する生物種: GINKGO BILOBA (MAIDENHAIR TREE)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 25903.82

構造登録者

Nanao, M.H.,Sayou, C.,Dumas, R.,Parcy, F. (登録日: 2014-12-10, 公開日: 2016-03-02, 最終更新日: 2024-05-08)

主引用文献

Sayou, C.,Nanao, M.H.,Jamin, M.,Pose, D.,Thevenon, E.,Gregoire, L.,Tichtinsky, G.,Denay, G.,Ott, F.,Peirats Llobet, M.,Schmid, M.,Dumas, R.,Parcy, F.
A Sam Oligomerization Domain Shapes the Genomic Binding Landscape of the Leafy Transcription Factor
Nat.Commun., 7:11222-, 2016

PubMed Abstract: Deciphering the mechanisms directing transcription factors (TFs) to specific genome regions is essential to understand and predict transcriptional regulation. TFs recognize short DNA motifs primarily through their DNA-binding domain. Some TFs also possess an oligomerization domain suspected to potentiate DNA binding but for which the genome-wide influence remains poorly understood. Here we focus on the LEAFY transcription factor, a master regulator of flower development in angiosperms. We have determined the crystal structure of its conserved amino-terminal domain, revealing an unanticipated Sterile Alpha Motif oligomerization domain. We show that this domain is essential to LEAFY floral function. Moreover, combined biochemical and genome-wide assays suggest that oligomerization is required for LEAFY to access regions with low-affinity binding sites or closed chromatin. This finding shows that domains that do not directly contact DNA can nevertheless have a profound impact on the DNA binding landscape of a TF.

PubMed: 27097556
DOI: 10.1038/NCOMMS11222

実験手法

X-RAY DIFFRACTION (2.25 Å)