4U84

Human Pin1 with S-hydroxyl-cysteine 113

Summary for 4U84

• Entry DOI: 10.2210/pdb4u84/pdb
• Related: 4U85 4U86
• Descriptor: Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, POLYETHYLENE GLYCOL (N=34) (3 entities in total)
• Functional Keywords: pin1 oxidation isomerase, isomerase
• Biological source: Homo sapiens (Human)
• Cellular location: Nucleus : Q13526
• Total number of polymer chains: 1
• Total formula weight: 21756.24

Authors

Li, W.,Zhang, Y. (deposition date: 2014-08-01, release date: 2015-04-08, Last modification date: 2024-11-06)

Primary citation

Chen, C.H.,Li, W.,Sultana, R.,You, M.H.,Kondo, A.,Shahpasand, K.,Kim, B.M.,Luo, M.L.,Nechama, M.,Lin, Y.M.,Yao, Y.,Lee, T.H.,Zhou, X.Z.,Swomley, A.M.,Allan Butterfield, D.,Zhang, Y.,Lu, K.P.
Pin1 cysteine-113 oxidation inhibits its catalytic activity and cellular function in Alzheimer's disease.
Neurobiol.Dis., 76:13-23, 2015

PubMed Abstract: The unique proline isomerase Pin1 is pivotal for protecting against age-dependent neurodegeneration in Alzheimer's disease (AD), with its inhibition providing a molecular link between tangle and plaque pathologies. Pin1 is oxidatively modified in human AD brains, but little is known about its regulatory mechanisms and pathological significance of such Pin1 modification. In this paper, our determination of crystal structures of oxidized Pin1 reveals a series of Pin1 oxidative modifications on Cys113 in a sequential fashion. Cys113 oxidization is further confirmed by generating antibodies specifically recognizing oxidized Cys113 of Pin1. Furthermore, Pin1 oxidation on Cys113 inactivates its catalytic activity in vitro, and Ala point substitution of Cys113 inactivates the ability of Pin1 to isomerize tau as well as to promote protein turnover of tau and APP. Moreover, redox regulation affects Pin1 subcellular localization and Pin1-mediated neuronal survival in response to hypoxia treatment. Importantly, Cys113-oxidized Pin1 is significantly increased in human AD brain comparing to age-matched controls. These results not only identify a novel Pin1 oxidation site to be the critical catalytic residue Cys113, but also provide a novel oxidative regulation mechanism for inhibiting Pin1 activity in AD. These results suggest that preventing Pin1 oxidization might help to reduce the risk of AD.

PubMed: 25576397
DOI: 10.1016/j.nbd.2014.12.027

Experimental method

X-RAY DIFFRACTION (1.78 Å)