4U63

Crystal structure of a bacterial class III photolyase from Agrobacterium tumefaciens at 1.67A resolution

4U63 の概要

• エントリーDOI: 10.2210/pdb4u63/pdb
• 分子名称: DNA photolyase, 5,10-METHENYL-6,7,8-TRIHYDROFOLIC ACID, FLAVIN-ADENINE DINUCLEOTIDE, ... (6 entities in total)
• 機能のキーワード: photolyase, dna repair, lyase, methenyltetrahydrofolate, cyclopyrimidine dimer, flavin, fad, flavoprotein, photoreduction, trp triade, double-stranded, dna damage, dna, ultraviolet rays, agrobacterium tumefaciens
• 由来する生物種: Agrobacterium tumefaciens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 58748.56

構造登録者

Scheerer, P.,Zhang, F.,Kalms, J.,von Stetten, D.,Krauss, N.,Oberpichler, I.,Lamparter, T. (登録日: 2014-07-26, 公開日: 2015-03-25, 最終更新日: 2023-12-20)

主引用文献

Scheerer, P.,Zhang, F.,Kalms, J.,von Stetten, D.,Krau, N.,Oberpichler, I.,Lamparter, T.
The Class III Cyclobutane Pyrimidine Dimer Photolyase Structure Reveals a New Antenna Chromophore Binding Site and Alternative Photoreduction Pathways.
J.Biol.Chem., 290:11504-11514, 2015

PubMed Abstract: Photolyases are proteins with an FAD chromophore that repair UV-induced pyrimidine dimers on the DNA in a light-dependent manner. The cyclobutane pyrimidine dimer class III photolyases are structurally unknown but closely related to plant cryptochromes, which serve as blue-light photoreceptors. Here we present the crystal structure of a class III photolyase termed photolyase-related protein A (PhrA) of Agrobacterium tumefaciens at 1.67-Å resolution. PhrA contains 5,10-methenyltetrahydrofolate (MTHF) as an antenna chromophore with a unique binding site and mode. Two Trp residues play pivotal roles for stabilizing MTHF by a double π-stacking sandwich. Plant cryptochrome I forms a pocket at the same site that could accommodate MTHF or a similar molecule. The PhrA structure and mutant studies showed that electrons flow during FAD photoreduction proceeds via two Trp triads. The structural studies on PhrA give a clearer picture on the evolutionary transition from photolyase to photoreceptor.

PubMed: 25784552
DOI: 10.1074/jbc.M115.637868

実験手法

X-RAY DIFFRACTION (1.67 Å)