4U5A

Sporozoite Protein for Cell Traversal

4U5A の概要

• エントリーDOI: 10.2210/pdb4u5a/pdb
• 分子名称: Sporozoite microneme protein essential for cell traversal (1 entity in total)
• 機能のキーワード: four helix bundle, transport protein
• 由来する生物種: Plasmodium berghei
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 92861.61

構造登録者

Hamaoka, B.Y. (登録日: 2014-07-25, 公開日: 2014-12-24, 最終更新日: 2023-12-27)

主引用文献

Hamaoka, B.Y.,Ghosh, P.
Structure of the Essential Plasmodium Host Cell Traversal Protein SPECT1.
Plos One, 9:e114685-e114685, 2014

PubMed Abstract: Host cell traversal by Plasmodium, the protozoan cause of malaria, is an essential part of this parasite's virulence. In this process, the parasite enters a host cell through a parasite-induced pore, traverses the host cell, and then exits the host cell. Two P. berghei proteins, SPECT1 and SPECT2, are required for host cell traversal by the sporozoite form of the parasite. In the absence of either, no pore formation is observed. While SPECT2 has sequence homology to pore-forming proteins, SPECT1 has no homology to proteins of known structure or function. Here we present the 2.75 Å resolution structure of a slightly truncated version of P. berghei SPECT1. The structure reveals that the protein forms a four-helix bundle, with the rare feature of having all of these helices in parallel or antiparallel alignment. Also notable is the presence of a large, conserved, hydrophobic internal cavity in the protein, which may constitute a ligand-binding site or be indicative of partial instability in SPECT1, or both. The structure of SPECT1 will make possible targeted mutagenesis experiments aimed at understanding its mechanism of action in host cell traversal.

PubMed: 25479287
DOI: 10.1371/journal.pone.0114685

実験手法

X-RAY DIFFRACTION (2.75 Å)