4U0H

Crystal Structure of M. tuberculosis ClpP1P1

Summary for 4U0H

• Entry DOI: 10.2210/pdb4u0h/pdb
• Descriptor: ATP-dependent Clp protease proteolytic subunit 1, SULFATE ION (3 entities in total)
• Functional Keywords: hydrolase, peptidase
• Biological source: Mycobacterium tuberculosis
• Total number of polymer chains: 7
• Total formula weight: 151432.58

Authors

Schmitz, K.R.,Carney, D.W.,Sello, J.K.,Sauer, R.T. (deposition date: 2014-07-11, release date: 2014-10-08, Last modification date: 2024-10-16)

Primary citation

Schmitz, K.R.,Carney, D.W.,Sello, J.K.,Sauer, R.T.
Crystal structure of Mycobacterium tuberculosis ClpP1P2 suggests a model for peptidase activation by AAA+ partner binding and substrate delivery.
Proc.Natl.Acad.Sci.USA, 111:E4587-E4595, 2014

PubMed Abstract: Caseinolytic peptidase P (ClpP), a double-ring peptidase with 14 subunits, collaborates with ATPases associated with diverse activities (AAA+) partners to execute ATP-dependent protein degradation. Although many ClpP enzymes self-assemble into catalytically active homo-tetradecamers able to cleave small peptides, the Mycobacterium tuberculosis enzyme consists of discrete ClpP1 and ClpP2 heptamers that require a AAA+ partner and protein-substrate delivery or a peptide agonist to stabilize assembly of the active tetradecamer. Here, we show that cyclic acyldepsipeptides (ADEPs) and agonist peptides synergistically activate ClpP1P2 by mimicking AAA+ partners and substrates, respectively, and determine the structure of the activated complex. Our studies establish the basis of heteromeric ClpP1P2 assembly and function, reveal tight coupling between the conformations of each ring, show that ADEPs bind only to one ring but appear to open the axial pores of both rings, provide a foundation for rational drug development, and suggest strategies for studying the roles of individual ClpP1 and ClpP2 rings in Clp-family proteolysis.

PubMed: 25267638
DOI: 10.1073/pnas.1417120111

Experimental method

X-RAY DIFFRACTION (3.2479 Å)