4TZW

Co-crystals of the Ternary Complex Containing a T-box Stem I RNA, its Cognate tRNAGly, and B. subtilis YbxF protein, treated by removing lithium sulfate and replacing Mg2+ with Sr2+ post crystallization

4TZW の概要

• エントリーDOI: 10.2210/pdb4tzw/pdb
• 関連するPDBエントリー: 4LCK 4TZP 4TZV 4TZX 4TZY 4TZZ
• 分子名称: Ribosome-associated protein L7Ae-like, engineered tRNA, T-box Stem I, ... (4 entities in total)
• 機能のキーワード: rna, riboswitch, trna, t-box, ribosomal protein-rna complex, ribosomal protein/rna
• 由来する生物種: Bacillus subtilis; 詳細
• 細胞内の位置: Cytoplasm: P46350
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 69013.16

構造登録者

Zhang, J.,Ferre-D'Amare, A.R. (登録日: 2014-07-11, 公開日: 2014-09-10, 最終更新日: 2024-10-23)

主引用文献

Zhang, J.,Ferre-D'Amare, A.R.
Dramatic Improvement of Crystals of Large RNAs by Cation Replacement and Dehydration.
Structure, 22:1363-1371, 2014

PubMed Abstract: Compared to globular proteins, RNAs with complex 3D folds are characterized by poorly differentiated molecular surfaces dominated by backbone phosphates, sparse tertiary contacts stabilizing global architecture, and conformational flexibility. The resulting generally poor order of crystals of large RNAs and their complexes frequently hampers crystallographic structure determination. We describe and rationalize a postcrystallization treatment strategy that exploits the importance of solvation and counterions for RNA folding. Replacement of Li(+) and Mg(2+) needed for growth of crystals of a tRNA-riboswitch-protein complex with Sr(2+), coupled with dehydration, dramatically improved the resolution limit (8.5-3.2 Å) and data quality, enabling structure determination. The soft Sr(2+) ion forms numerous stabilizing intermolecular contacts. Comparison of pre- and posttreatment structures reveals how RNA assemblies redistribute as quasi-rigid bodies to yield improved crystal packing. Cation exchange complements previously reported postcrystallization dehydration of protein crystals and represents a potentially general strategy for improving crystals of large RNAs.

PubMed: 25185828
DOI: 10.1016/j.str.2014.07.011

実験手法

X-RAY DIFFRACTION (4.671 Å)