4TZT

CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS ENOYL REDUCTASE (INHA) COMPLEXED WITH N-(3-CHLORO-2-METHYLPHENYL)-1-CYCLOHEXYL- 5-OXOPYRROLIDINE-3-CARBOXAMIDE

Replaces:  2H7P

Summary for 4TZT

• Entry DOI: 10.2210/pdb4tzt/pdb
• Related: 4TRJ 4TZK 4U0J 4U0K
• Descriptor: Enoyl-[acyl-carrier-protein] reductase [NADH], NICOTINAMIDE-ADENINE-DINUCLEOTIDE, (3S)-N-(3-CHLORO-2-METHYLPHENYL)-1-CYCLOHEXYL-5-OXOPYRROLIDINE-3-CARBOXAMIDE, ... (4 entities in total)
• Functional Keywords: oxidoreductase, pyrrolidine carboxamide
• Biological source: Mycobacterium tuberculosis
• Total number of polymer chains: 1
• Total formula weight: 29553.05

Authors

He, X.,Alian, A.,Stroud, R.M.,Ortiz de Montellano, P.R. (deposition date: 2014-07-10, release date: 2014-08-20, Last modification date: 2023-12-27)

Primary citation

He, X.,Alian, A.,Stroud, R.M.,Ortiz de Montellano, P.R.
Pyrrolidine carboxamides as a novel class of inhibitors of enoyl acyl carrier protein reductase from Mycobacterium tuberculosis
J. Med. Chem., :6308-6323, 2006

PubMed Abstract: In view of the worldwide spread of multidrug resistance of Mycobacterium tuberculosis, there is an urgent need to discover antituberculosis agent with novel structures. InhA, the enoyl acyl carrier protein reductase (ENR) from M. tuberculosis, is one of the key enzymes involved in the mycobacterial fatty acid elongation cycle and has been validated as an effective antimicrobial target. We report here the discovery, through high-throughput screening, of a series of pyrrolidine carboxamides as a novel class of potent InhA inhibitors. Crystal structures of InhA complexed with three inhibitors have been used to elucidate the inhibitor binding mode. The potency of the lead compound was improved over 160-fold by subsequent optimization through iterative microtiter library synthesis followed by in situ activity screening without purification. Resolution of racemic mixtures of several inhibitors indicate that only one enantiomer is active as an inhibitor of InhA.

PubMed: 17034137
DOI: 10.1021/JM060715Y

Experimental method

X-RAY DIFFRACTION (1.86 Å)