4TZL

Structure of C. elegans HTP-2 bound to HIM-3 closure motif, P21 form

4TZL の概要

• エントリーDOI: 10.2210/pdb4tzl/pdb
• 関連するPDBエントリー: 4TRK 4TZJ 4TZM 4TZN 4TZO 4TZQ 4TZS
• 分子名称: Protein HTP-2, C. elegans HIM-3 closure motif (3 entities in total)
• 機能のキーワード: horma domain, meiosis, chromosome axis, peptide binding protein
• 由来する生物種: Caenorhabditis elegans; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 61855.37

構造登録者

Rosenberg, S.C.,Corbett, K.D. (登録日: 2014-07-10, 公開日: 2014-11-19, 最終更新日: 2023-12-27)

主引用文献

Kim, Y.,Rosenberg, S.C.,Kugel, C.L.,Kostow, N.,Rog, O.,Davydov, V.,Su, T.Y.,Dernburg, A.F.,Corbett, K.D.
The Chromosome Axis Controls Meiotic Events through a Hierarchical Assembly of HORMA Domain Proteins.
Dev.Cell, 31:487-502, 2014

PubMed Abstract: Proteins of the HORMA domain family play central, but poorly understood, roles in chromosome organization and dynamics during meiosis. In Caenorhabditis elegans, four such proteins (HIM-3, HTP-1, HTP-2, and HTP-3) have distinct but overlapping functions. Through combined biochemical, structural, and in vivo analysis, we find that these proteins form hierarchical complexes through binding of their HORMA domains to cognate peptides within their partners' C-terminal tails, analogous to the "safety belt" binding mechanism of Mad2. These interactions are critical for recruitment of HIM-3, HTP-1, and HTP-2 to chromosome axes. HTP-3, in addition to recruiting the other HORMA domain proteins to the axis, plays an independent role in sister chromatid cohesion and double-strand break formation. Finally, we find that mammalian HORMAD1 binds a motif found both at its own C terminus and at that of HORMAD2, indicating that this mode of intermolecular association is a conserved feature of meiotic chromosome structure in eukaryotes.

PubMed: 25446517
DOI: 10.1016/j.devcel.2014.09.013

実験手法

X-RAY DIFFRACTION (2.537 Å)