4TVF

OxyB from Actinoplanes teichomyceticus

4TVF の概要

• エントリーDOI: 10.2210/pdb4tvf/pdb
• 分子名称: OxyB, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: cytochrome p450 phenolic coupling enzyme teicoplanin biosynthesis, oxidoreductase
• 由来する生物種: Actinoplanes teichomyceticus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48270.77

構造登録者

Haslinger, K.,Cryle, M.J. (登録日: 2014-06-26, 公開日: 2014-11-12, 最終更新日: 2023-12-20)

主引用文献

Haslinger, K.,Maximowitsch, E.,Brieke, C.,Koch, A.,Cryle, M.J.
Cytochrome P450 OxyBtei Catalyzes the First Phenolic Coupling Step in Teicoplanin Biosynthesis.
Chembiochem, 15:2719-2728, 2014

PubMed Abstract: Bacterial cytochrome P450s form a remarkable clade of the P450 superfamily of oxidative hemoproteins, and are often involved in the biosynthesis of complex natural products. Those in a subgroup known as "Oxy enzymes" play a crucial role in the biosynthesis of glycopeptide antibiotics, including vancomycin and teicoplanin. The Oxy enzymes catalyze crosslinking of aromatic residues in the non-ribosomal antibiotic precursor peptide while it remains bound to the non-ribosomal peptide synthetase (NRPS); this crosslinking secures the three-dimensional structure of the glycopeptide, crucial for antibiotic activity. We have characterized OxyBtei , the first of the Oxy enzymes in teicoplanin biosynthesis. Our results reveal that OxyBtei possesses a structure similar to those of other Oxy proteins and is active in crosslinking NRPS-bound peptide substrates. However, OxyBtei displays a significantly altered activity spectrum against peptide substrates compared to its well-studied vancomycin homologue.

PubMed: 25358800
DOI: 10.1002/cbic.201402441

実験手法

X-RAY DIFFRACTION (1.9 Å)