4TVC

N-terminally truncated dextransucrase DSR-E from Leuconostoc mesenteroides NRRL B-1299 in complex with gluco-oligosaccharides

4TVC の概要

• エントリーDOI: 10.2210/pdb4tvc/pdb
• 関連するPDBエントリー: 4TTU
• 分子名称: Dextransucrase, alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose, CALCIUM ION, ... (7 entities in total)
• 機能のキーワード: alpha-1, 2-branching-sucrase, glucan binding domain, gluco-oligosaccharides, glucansucrase, transferase
• 由来する生物種: Leuconostoc mesenteroides
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 124872.54

構造登録者

Brison, Y.,Remaud-Simeon, M.,Mourey, L.,Tranier, S. (登録日: 2014-06-26, 公開日: 2015-07-29, 最終更新日: 2024-11-20)

主引用文献

Brison, Y.,Malbert, Y.,Czaplicki, G.,Mourey, L.,Remaud-Simeon, M.,Tranier, S.
Structural Insights into the Carbohydrate Binding Ability of an alpha-(12) Branching Sucrase from Glycoside Hydrolase Family 70.
J.Biol.Chem., 291:7527-7540, 2016

PubMed Abstract: The α-(1→2) branching sucrase ΔN123-GBD-CD2 is a transglucosylase belonging to glycoside hydrolase family 70 (GH70) that catalyzes the transfer ofd-glucosyl units from sucroseto dextrans or gluco-oligosaccharides via the formation of α-(1→2) glucosidic linkages. The first structures of ΔN123-GBD-CD2 in complex withd-glucose, isomaltosyl, or isomaltotriosyl residues were solved. The glucose complex revealed three glucose-binding sites in the catalytic gorge and six additional binding sites at the surface of domains B, IV, and V. Soaking with isomaltotriose or gluco-oligosaccharides led to structures in which isomaltosyl or isomaltotriosyl residues were found in glucan binding pockets located in domain V. One aromatic residue is systematically identified at the bottom of these pockets in stacking interaction with one glucosyl moiety. The carbohydrate is also maintained by a network of hydrogen bonds and van der Waals interactions. The sequence of these binding pockets is conserved and repeatedly present in domain V of several GH70 glucansucrases known to bind α-glucans. These findings provide the first structural evidence of the molecular interaction occurring between isomalto-oligosaccharides and domain V of the GH70 enzymes.

PubMed: 26865636
DOI: 10.1074/jbc.M115.688796

実験手法

X-RAY DIFFRACTION (1.85 Å)