4TTZ

N-terminal domain of C. Reinhardtii SAS-6 homolog bld12p variant G94E F145W Q147K (NN26)

4TTZ の概要

• エントリーDOI: 10.2210/pdb4ttz/pdb
• 分子名称: Centriole protein (2 entities in total)
• 機能のキーワード: stabilization, centriole sas-6, cartwheel, structural protein, beta-sandwich, alpha-beta protein, centriolar
• 由来する生物種: Chlamydomonas reinhardtii
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 72863.07

構造登録者

Hilbert, M.,Kraatz, S.H.W. (登録日: 2014-06-23, 公開日: 2015-07-01, 最終更新日: 2023-12-20)

主引用文献

Hilbert, M.,Noga, A.,Frey, D.,Hamel, V.,Guichard, P.,Kraatz, S.H.,Pfreundschuh, M.,Hosner, S.,Fluckiger, I.,Jaussi, R.,Wieser, M.M.,Thieltges, K.M.,Deupi, X.,Muller, D.J.,Kammerer, R.A.,Gonczy, P.,Hirono, M.,Steinmetz, M.O.
SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture.
Nat.Cell Biol., 18:393-403, 2016

PubMed Abstract: Centrioles are critical for the formation of centrosomes, cilia and flagella in eukaryotes. They are thought to assemble around a nine-fold symmetric cartwheel structure established by SAS-6 proteins. Here, we have engineered Chlamydomonas reinhardtii SAS-6-based oligomers with symmetries ranging from five- to ten-fold. Expression of a SAS-6 mutant that forms six-fold symmetric cartwheel structures in vitro resulted in cartwheels and centrioles with eight- or nine-fold symmetries in vivo. In combination with Bld10 mutants that weaken cartwheel-microtubule interactions, this SAS-6 mutant produced six- to eight-fold symmetric cartwheels. Concurrently, the microtubule wall maintained eight- and nine-fold symmetries. Expressing SAS-6 with analogous mutations in human cells resulted in nine-fold symmetric centrioles that exhibited impaired length and organization. Together, our data suggest that the self-assembly properties of SAS-6 instruct cartwheel symmetry, and lead us to propose a model in which the cartwheel and the microtubule wall assemble in an interdependent manner to establish the native architecture of centrioles.

PubMed: 26999736
DOI: 10.1038/ncb3329

実験手法

X-RAY DIFFRACTION (2.5 Å)