4TS0

Crystal structure of the Spinach RNA aptamer in complex with DFHBI, barium ions

4TS0 の概要

• エントリーDOI: 10.2210/pdb4ts0/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900003
• 分子名称: Spinach RNA aptamer, bimolecular construct, beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose, POTASSIUM ION, ... (7 entities in total)
• 機能のキーワード: aptamer, fluorescence, g-quadruplex, rna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31765.24

構造登録者

Warner, K.D.,Chen, M.C.,Song, W.,Strack, R.L.,Thorn, A.,Jaffrey, S.R.,Ferre-D'Amare, A.R. (登録日: 2014-06-18, 公開日: 2014-07-23, 最終更新日: 2023-12-27)

主引用文献

Warner, K.D.,Chen, M.C.,Song, W.,Strack, R.L.,Thorn, A.,Jaffrey, S.R.,Ferre-D'Amare, A.R.
Structural basis for activity of highly efficient RNA mimics of green fluorescent protein.
Nat.Struct.Mol.Biol., 21:658-663, 2014

PubMed Abstract: GFP and its derivatives revolutionized the study of proteins. Spinach is a recently reported in vitro-evolved RNA mimic of GFP, which as genetically encoded fusions makes possible live-cell, real-time imaging of biological RNAs without resorting to large RNA-binding protein-GFP fusions. To elucidate the molecular basis of Spinach fluorescence, we solved the cocrystal structure of Spinach bound to its cognate exogenous chromophore, showing that Spinach activates the small molecule by immobilizing it between a base triple, a G-quadruplex and an unpaired G. Mutational and NMR analyses indicate that the G-quadruplex is essential for Spinach fluorescence, is also present in other fluorogenic RNAs and may represent a general strategy for RNAs to induce fluorescence of chromophores. The structure guided the design of a miniaturized 'Baby Spinach', and it provides a foundation for structure-driven design and tuning of fluorescent RNAs.

PubMed: 25026079
DOI: 10.1038/nsmb.2865

実験手法

X-RAY DIFFRACTION (2.8 Å)