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4TNL

1.8 A resolution room temperature structure of Thermolysin recorded using an XFEL

Summary for 4TNL
Entry DOI10.2210/pdb4tnl/pdb
DescriptorThermolysin, ZINC ION, CALCIUM ION, ... (4 entities in total)
Functional Keywordszn protease, x-ray free electron laser, hydrolase
Biological sourceBacillus thermoproteolyticus
Cellular locationSecreted: P00800
Total number of polymer chains1
Total formula weight34586.06
Authors
Primary citationKern, J.,Tran, R.,Alonso-Mori, R.,Koroidov, S.,Echols, N.,Hattne, J.,Ibrahim, M.,Gul, S.,Laksmono, H.,Sierra, R.G.,Gildea, R.J.,Han, G.,Hellmich, J.,Lassalle-Kaiser, B.,Chatterjee, R.,Brewster, A.S.,Stan, C.A.,Glockner, C.,Lampe, A.,DiFiore, D.,Milathianaki, D.,Fry, A.R.,Seibert, M.M.,Koglin, J.E.,Gallo, E.,Uhlig, J.,Sokaras, D.,Weng, T.C.,Zwart, P.H.,Skinner, D.E.,Bogan, M.J.,Messerschmidt, M.,Glatzel, P.,Williams, G.J.,Boutet, S.,Adams, P.D.,Zouni, A.,Messinger, J.,Sauter, N.K.,Bergmann, U.,Yano, J.,Yachandra, V.K.
Taking snapshots of photosynthetic water oxidation using femtosecond X-ray diffraction and spectroscopy.
Nat Commun, 5:4371-4371, 2014
Cited by
PubMed Abstract: The dioxygen we breathe is formed by light-induced oxidation of water in photosystem II. O2 formation takes place at a catalytic manganese cluster within milliseconds after the photosystem II reaction centre is excited by three single-turnover flashes. Here we present combined X-ray emission spectra and diffraction data of 2-flash (2F) and 3-flash (3F) photosystem II samples, and of a transient 3F' state (250 μs after the third flash), collected under functional conditions using an X-ray free electron laser. The spectra show that the initial O-O bond formation, coupled to Mn reduction, does not yet occur within 250 μs after the third flash. Diffraction data of all states studied exhibit an anomalous scattering signal from Mn but show no significant structural changes at the present resolution of 4.5 Å. This study represents the initial frames in a molecular movie of the structural changes during the catalytic reaction in photosystem II.
PubMed: 25006873
DOI: 10.1038/ncomms5371
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.8 Å)
Structure validation

237992

數據於2025-06-25公開中

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