4TNL

1.8 A resolution room temperature structure of Thermolysin recorded using an XFEL

4TNL の概要

• エントリーDOI: 10.2210/pdb4tnl/pdb
• 分子名称: Thermolysin, ZINC ION, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: zn protease, x-ray free electron laser, hydrolase
• 由来する生物種: Bacillus thermoproteolyticus
• 細胞内の位置: Secreted: P00800
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34586.06

構造登録者

Kern, J.,Tran, R.,Alonso-Mori, R.,Koroidov, S.,Echols, N.,Hattne, J.,Ibrahim, M.,Gul, S.,Laksmono, H.,Sierra, R.G.,Gildea, R.J.,Han, G.,Hellmich, J.,Lassalle-Kaiser, B.,Chatterjee, R.,Brewster, A.,Stan, C.A.,Gloeckner, C.,Lampe, A.,DiFiore, D.,Milathianaki, D.,Fry, A.R.,Seibert, M.M.,Koglin, J.E.,Gallo, E.,Uhlig, J.,Sokaras, D.,Weng, T.-C.,Zwart, P.H.,Skinner, D.E.,Bogan, M.J.,Messerschmidt, M.,Glatzel, P.,Williams, G.J.,Boutet, S.,Adams, P.D.,Zouni, A.,Messinger, J.,Sauter, N.K.,Bergmann, U.,Yano, J.,Yachandra, V.K. (登録日: 2014-06-04, 公開日: 2014-07-09, 最終更新日: 2023-09-27)

主引用文献

Kern, J.,Tran, R.,Alonso-Mori, R.,Koroidov, S.,Echols, N.,Hattne, J.,Ibrahim, M.,Gul, S.,Laksmono, H.,Sierra, R.G.,Gildea, R.J.,Han, G.,Hellmich, J.,Lassalle-Kaiser, B.,Chatterjee, R.,Brewster, A.S.,Stan, C.A.,Glockner, C.,Lampe, A.,DiFiore, D.,Milathianaki, D.,Fry, A.R.,Seibert, M.M.,Koglin, J.E.,Gallo, E.,Uhlig, J.,Sokaras, D.,Weng, T.C.,Zwart, P.H.,Skinner, D.E.,Bogan, M.J.,Messerschmidt, M.,Glatzel, P.,Williams, G.J.,Boutet, S.,Adams, P.D.,Zouni, A.,Messinger, J.,Sauter, N.K.,Bergmann, U.,Yano, J.,Yachandra, V.K.
Taking snapshots of photosynthetic water oxidation using femtosecond X-ray diffraction and spectroscopy.
Nat Commun, 5:4371-4371, 2014

PubMed Abstract: The dioxygen we breathe is formed by light-induced oxidation of water in photosystem II. O2 formation takes place at a catalytic manganese cluster within milliseconds after the photosystem II reaction centre is excited by three single-turnover flashes. Here we present combined X-ray emission spectra and diffraction data of 2-flash (2F) and 3-flash (3F) photosystem II samples, and of a transient 3F' state (250 μs after the third flash), collected under functional conditions using an X-ray free electron laser. The spectra show that the initial O-O bond formation, coupled to Mn reduction, does not yet occur within 250 μs after the third flash. Diffraction data of all states studied exhibit an anomalous scattering signal from Mn but show no significant structural changes at the present resolution of 4.5 Å. This study represents the initial frames in a molecular movie of the structural changes during the catalytic reaction in photosystem II.

PubMed: 25006873
DOI: 10.1038/ncomms5371

実験手法

X-RAY DIFFRACTION (1.8 Å)