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4TNH

RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution

4TNH の概要
エントリーDOI10.2210/pdb4tnh/pdb
関連するPDBエントリー4TNI 4TNJ 4TNK
分子名称Photosystem Q(B) protein 1, Photosystem II reaction center protein K, Photosystem II reaction center protein L, ... (35 entities in total)
機能のキーワードphotosynthesis, water oxidation, membrane protein, x-ray free electron laser, electron transport
由来する生物種Thermosynechococcus elongatus
詳細
タンパク質・核酸の鎖数40
化学式量合計762724.63
構造登録者
主引用文献Kern, J.,Tran, R.,Alonso-Mori, R.,Koroidov, S.,Echols, N.,Hattne, J.,Ibrahim, M.,Gul, S.,Laksmono, H.,Sierra, R.G.,Gildea, R.J.,Han, G.,Hellmich, J.,Lassalle-Kaiser, B.,Chatterjee, R.,Brewster, A.S.,Stan, C.A.,Glockner, C.,Lampe, A.,DiFiore, D.,Milathianaki, D.,Fry, A.R.,Seibert, M.M.,Koglin, J.E.,Gallo, E.,Uhlig, J.,Sokaras, D.,Weng, T.C.,Zwart, P.H.,Skinner, D.E.,Bogan, M.J.,Messerschmidt, M.,Glatzel, P.,Williams, G.J.,Boutet, S.,Adams, P.D.,Zouni, A.,Messinger, J.,Sauter, N.K.,Bergmann, U.,Yano, J.,Yachandra, V.K.
Taking snapshots of photosynthetic water oxidation using femtosecond X-ray diffraction and spectroscopy.
Nat Commun, 5:4371-4371, 2014
Cited by
PubMed Abstract: The dioxygen we breathe is formed by light-induced oxidation of water in photosystem II. O2 formation takes place at a catalytic manganese cluster within milliseconds after the photosystem II reaction centre is excited by three single-turnover flashes. Here we present combined X-ray emission spectra and diffraction data of 2-flash (2F) and 3-flash (3F) photosystem II samples, and of a transient 3F' state (250 μs after the third flash), collected under functional conditions using an X-ray free electron laser. The spectra show that the initial O-O bond formation, coupled to Mn reduction, does not yet occur within 250 μs after the third flash. Diffraction data of all states studied exhibit an anomalous scattering signal from Mn but show no significant structural changes at the present resolution of 4.5 Å. This study represents the initial frames in a molecular movie of the structural changes during the catalytic reaction in photosystem II.
PubMed: 25006873
DOI: 10.1038/ncomms5371
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (4.90000680874 Å)
構造検証レポート
Validation report summary of 4tnh
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-09に公開中

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