4TLM

Crystal structure of GluN1/GluN2B NMDA receptor, structure 2

4TLM の概要

• エントリーDOI: 10.2210/pdb4tlm/pdb
• 関連するPDBエントリー: 4TLL
• 分子名称: receptor subunit GluN1, receptor subunit GluN2B, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: neurotransmitter receptor, nmda receptor, glun1/glun2b, membrane protein, ion channel, signaling protein
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 374109.17

構造登録者

Gouaux, E.,Lee, C.-H.,Lu, W. (登録日: 2014-05-30, 公開日: 2014-07-02, 最終更新日: 2023-12-27)

主引用文献

Lee, C.H.,Lu, W.,Michel, J.C.,Goehring, A.,Du, J.,Song, X.,Gouaux, E.
NMDA receptor structures reveal subunit arrangement and pore architecture.
Nature, 511:191-197, 2014

PubMed Abstract: N-methyl-d-aspartate (NMDA) receptors are Hebbian-like coincidence detectors, requiring binding of glycine and glutamate in combination with the relief of voltage-dependent magnesium block to open an ion conductive pore across the membrane bilayer. Despite the importance of the NMDA receptor in the development and function of the brain, a molecular structure of an intact receptor has remained elusive. Here we present X-ray crystal structures of the Xenopus laevis GluN1-GluN2B NMDA receptor with the allosteric inhibitor, Ro25-6981, partial agonists and the ion channel blocker, MK-801. Receptor subunits are arranged in a 1-2-1-2 fashion, demonstrating extensive interactions between the amino-terminal and ligand-binding domains. The transmembrane domains harbour a closed-blocked ion channel, a pyramidal central vestibule lined by residues implicated in binding ion channel blockers and magnesium, and a ∼twofold symmetric arrangement of ion channel pore loops. These structures provide new insights into the architecture, allosteric coupling and ion channel function of NMDA receptors.

PubMed: 25008524
DOI: 10.1038/nature13548

実験手法

X-RAY DIFFRACTION (3.77 Å)