4TL1

GCN4-p1 with mutation to 1-Aminocyclohexanecarboxylic acid at residue 10

4TL1 の概要

• エントリーDOI: 10.2210/pdb4tl1/pdb
• 分子名称: General control protein GCN4, ISOPROPYL ALCOHOL (3 entities in total)
• 機能のキーワード: coiled coil, transcription
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 8111.62

構造登録者

Tavenor, N.A.,Silva, K.I.,Saxena, S.,Horne, W.S. (登録日: 2014-05-28, 公開日: 2014-08-06, 最終更新日: 2023-09-27)

主引用文献

Tavenor, N.A.,Silva, K.I.,Saxena, S.,Horne, W.S.
Origins of Structural Flexibility in Protein-Based Supramolecular Polymers Revealed by DEER Spectroscopy.
J.Phys.Chem.B, 118:9881-9889, 2014

PubMed Abstract: Modular assembly of bio-inspired supramolecular polymers is a powerful technique to develop new soft nanomaterials, and protein folding is a versatile basis for preparing such materials. Previous work demonstrated a significant difference in the physical properties of closely related supramolecular polymers composed of building blocks in which identical coiled-coil-forming peptides are cross-linked by one of two subtly different organic linkers (one flexible and the other rigid). Herein, we investigate the molecular basis for this observation by isolating a single subunit of the supramolecular polymer chain and probing its structure and conformational flexibility by double electron-electron resonance (DEER) spectroscopy. Experimental spin-spin distance distributions for two different labeling sites coupled with molecular dynamics simulations provide insights into how the linker structure impacts chain dynamics in the coiled-coil supramolecular polymer.

PubMed: 25060334
DOI: 10.1021/jp505643w

実験手法

X-RAY DIFFRACTION (1.8 Å)