4S2N

OXA-48 in complex with Avibactam at pH 8.5

4S2N の概要

• エントリーDOI: 10.2210/pdb4s2n/pdb
• 分子名称: Beta-lactamase, (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide, ... (4 entities in total)
• 機能のキーワード: hydrolase-antibiotic complex, hydrolase/antibiotic
• 由来する生物種: Klebsiella pneumoniae; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 122741.93

構造登録者

King, D.T.,Strynadka, N.C.J. (登録日: 2015-01-21, 公開日: 2015-02-25, 最終更新日: 2023-12-06)

主引用文献

King, D.T.,King, A.M.,Lal, S.M.,Wright, G.D.,Strynadka, N.C.
Molecular Mechanism of Avibactam-Mediated beta-Lactamase Inhibition.
ACS Infect Dis, 1:175-184, 2015

PubMed Abstract: Emerging β-lactamase-mediated resistance is threatening the clinical utility of the single most prominent class of antibacterial agents used in medicine, the β-lactams. The diazabicyclooctane avibactam is able to inhibit a wider range of serine β-lactamases than has been previously observed with β-lactamase inhibitors such as the widely prescribed clavulanic acid. However, despite its broad-spectrum activity, variable levels of inhibition have been observed for molecular class D β-lactamases. In order to better understand the molecular basis and spectrum of inhibition by avibactam, we provide structural and mechanistic analysis of the compound in complex with important class A and D serine β-lactamases. Herein, we reveal the 1.7- and 2.0-Å-resolution crystal structures of avibactam covalently bound to class D β-lactamases OXA-10 and OXA-48. Furthermore, a kinetic analysis of key active-site mutants for class A β-lactamase CTX-M-15 allows us to propose a validated mechanism for avibactam-mediated β-lactamase inhibition including a unique role for S130, which acts as a general base. This study provides molecular insights that will aid in the design and development of avibactam-based chemotherapeutic agents effective against emerging drug-resistant microorganisms.

PubMed: 27622530
DOI: 10.1021/acsinfecdis.5b00007

実験手法

X-RAY DIFFRACTION (2 Å)