4RX6

Structure of B. subtilis GlnK-ATP complex to 2.6 Angstrom

4RX6 の概要

• エントリーDOI: 10.2210/pdb4rx6/pdb
• 関連するPDBエントリー: 4R25
• 分子名称: Nitrogen regulatory PII-like protein, ADENOSINE-5'-TRIPHOSPHATE (3 entities in total)
• 機能のキーワード: pii protein, transcription
• 由来する生物種: Bacillus subtilis subsp. subtilis
• 細胞内の位置: Cell membrane ; Peripheral membrane protein : Q07428
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 41725.79

構造登録者

Schumacher, M.A.,Cuthbert, B.,Tonthat, N.,Chinnam, N.G.,Whitfill, T. (登録日: 2014-12-09, 公開日: 2015-12-30, 最終更新日: 2023-09-20)

主引用文献

Schumacher, M.A.,Chinnam, N.B.,Cuthbert, B.,Tonthat, N.K.,Whitfill, T.
Structures of regulatory machinery reveal novel molecular mechanisms controlling B. subtilis nitrogen homeostasis.
Genes Dev., 29:451-464, 2015

PubMed Abstract: All cells must sense and adapt to changing nutrient availability. However, detailed molecular mechanisms coordinating such regulatory pathways remain poorly understood. In Bacillus subtilis, nitrogen homeostasis is controlled by a unique circuitry composed of the regulator TnrA, which is deactivated by feedback-inhibited glutamine synthetase (GS) during nitrogen excess and stabilized by GlnK upon nitrogen depletion, and the repressor GlnR. Here we describe a complete molecular dissection of this network. TnrA and GlnR, the global nitrogen homeostatic transcription regulators, are revealed as founders of a new structural family of dimeric DNA-binding proteins with C-terminal, flexible, effector-binding sensors that modulate their dimerization. Remarkably, the TnrA sensor domains insert into GS intersubunit catalytic pores, destabilizing the TnrA dimer and causing an unprecedented GS dodecamer-to-tetradecamer conversion, which concomitantly deactivates GS. In contrast, each subunit of the GlnK trimer "templates" active TnrA dimers. Unlike TnrA, GlnR sensors mediate an autoinhibitory dimer-destabilizing interaction alleviated by GS, which acts as a GlnR chaperone. Thus, these studies unveil heretofore unseen mechanisms by which inducible sensor domains drive metabolic reprograming in the model Gram-positive bacterium B. subtilis.

PubMed: 25691471
DOI: 10.1101/gad.254714.114

実験手法

X-RAY DIFFRACTION (2.5994 Å)