4RX2

A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis

4RX2 の概要

• エントリーDOI: 10.2210/pdb4rx2/pdb
• 関連するPDBエントリー: 4RVA
• 分子名称: Beta-lactamase TEM, SULFATE ION (3 entities in total)
• 機能のキーワード: globular, beta-lactamase, hydrolase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 231831.42

構造登録者

Stojanoski, V.,Chow, D.,Hu, L.,Sankaran, B.,Gilbert, H.,Prasad, B.V.V.,Palzkill, T. (登録日: 2014-12-08, 公開日: 2015-03-04, 最終更新日: 2024-11-20)

主引用文献

Stojanoski, V.,Chow, D.C.,Hu, L.,Sankaran, B.,Gilbert, H.F.,Prasad, B.V.,Palzkill, T.
A Triple Mutant in the Omega-loop of TEM-1 beta-Lactamase Changes the Substrate Profile via a Large Conformational Change and an Altered General Base for Catalysis.
J.Biol.Chem., 290:10382-10394, 2015

PubMed Abstract: β-Lactamases are bacterial enzymes that hydrolyze β-lactam antibiotics. TEM-1 is a prevalent plasmid-encoded β-lactamase in Gram-negative bacteria that efficiently catalyzes the hydrolysis of penicillins and early cephalosporins but not oxyimino-cephalosporins. A previous random mutagenesis study identified a W165Y/E166Y/P167G triple mutant that displays greatly altered substrate specificity with increased activity for the oxyimino-cephalosporin, ceftazidime, and decreased activity toward all other β-lactams tested. Surprisingly, this mutant lacks the conserved Glu-166 residue critical for enzyme function. Ceftazidime contains a large, bulky side chain that does not fit optimally in the wild-type TEM-1 active site. Therefore, it was hypothesized that the substitutions in the mutant expand the binding site in the enzyme. To investigate structural changes and address whether there is an enlargement in the active site, the crystal structure of the triple mutant was solved to 1.44 Å. The structure reveals a large conformational change of the active site Ω-loop structure to create additional space for the ceftazidime side chain. The position of the hydroxyl group of Tyr-166 and an observed shift in the pH profile of the triple mutant suggests that Tyr-166 participates in the hydrolytic mechanism of the enzyme. These findings indicate that the highly conserved Glu-166 residue can be substituted in the mechanism of serine β-lactamases. The results reveal that the robustness of the overall β-lactamase fold coupled with the plasticity of an active site loop facilitates the evolution of enzyme specificity and mechanism.

PubMed: 25713062
DOI: 10.1074/jbc.M114.633438

実験手法

X-RAY DIFFRACTION (2.315 Å)