4RVZ

Crystal structure of tRNA fluorescent labeling enzyme

4RVZ の概要

• エントリーDOI: 10.2210/pdb4rvz/pdb
• 分子名称: tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS, ZINC ION, PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER, ... (5 entities in total)
• 機能のキーワード: trna modification, phosphorylation, tias, ligase
• 由来する生物種: Archaeoglobus fulgidus
• 細胞内の位置: Cytoplasm : O28025
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 49162.86

構造登録者

Dong, J.,Li, F.,Wang, J.,Gong, W. (登録日: 2014-11-29, 公開日: 2015-03-11, 最終更新日: 2024-11-27)

主引用文献

Li, F.,Dong, J.,Hu, X.,Gong, W.,Li, J.,Shen, J.,Tian, H.,Wang, J.
A covalent approach for site-specific RNA labeling in Mammalian cells.
Angew.Chem.Int.Ed.Engl., 54:4597-4602, 2015

PubMed Abstract: Advances in RNA research and RNA nanotechnology depend on the ability to manipulate and probe RNA with high precision through chemical approaches, both in vitro and in mammalian cells. However, covalent RNA labeling methods with scope and versatility comparable to those of current protein labeling strategies are underdeveloped. A method is reported for the site- and sequence-specific covalent labeling of RNAs in mammalian cells by using tRNA(Ile2) -agmatidine synthetase (Tias) and click chemistry. The crystal structure of Tias in complex with an azide-bearing agmatine analogue was solved to unravel the structural basis for Tias/substrate recognition. The unique RNA sequence specificity and plastic Tias/substrate recognition enable the site-specific transfer of azide/alkyne groups to an RNA molecule of interest in vitro and in mammalian cells. Subsequent click chemistry reactions facilitate the versatile labeling, functionalization, and visualization of target RNA.

PubMed: 25694369
DOI: 10.1002/anie.201410433

実験手法

X-RAY DIFFRACTION (2.9 Å)