4RUM

Crystal structure of the NiCo transition-metal riboswitch bound to cobalt

4RUM の概要

• エントリーDOI: 10.2210/pdb4rum/pdb
• 分子名称: NiCo riboswitch RNA, POTASSIUM ION, COBALT (II) ION, ... (7 entities in total)
• 機能のキーワード: rna helix, ligand sensor, cobalt and nickel binding, rna, riboswitch
• 由来する生物種: Synthetic
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31377.97

構造登録者

Ramesh, A.,Winkler, W.C. (登録日: 2014-11-21, 公開日: 2015-04-08, 最終更新日: 2024-02-28)

主引用文献

Furukawa, K.,Ramesh, A.,Zhou, Z.,Weinberg, Z.,Vallery, T.,Winkler, W.C.,Breaker, R.R.
Bacterial riboswitches cooperatively bind Ni(2+) or Co(2+) ions and control expression of heavy metal transporters.
Mol.Cell, 57:1088-1098, 2015

PubMed Abstract: Bacteria regularly encounter widely varying metal concentrations in their surrounding environment. As metals become depleted or, conversely, accrue to toxicity, microbes will activate cellular responses that act to maintain metal homeostasis. A suite of metal-sensing regulatory ("metalloregulatory") proteins orchestrate these responses by allosterically coupling the selective binding of target metals to the activity of DNA-binding domains. However, we report here the discovery, validation, and structural details of a widespread class of riboswitch RNAs, whose members selectively and tightly bind the low-abundance transition metals, Ni(2+) and Co(2+). These riboswitches bind metal cooperatively, and with affinities in the low micromolar range. The structure of a Co(2+)-bound RNA reveals a network of molecular contacts that explains how it achieves cooperative binding between adjacent sites. These findings reveal that bacteria have evolved to utilize highly selective metalloregulatory riboswitches, in addition to metalloregulatory proteins, for detecting and responding to toxic levels of heavy metals.

PubMed: 25794617
DOI: 10.1016/j.molcel.2015.02.009

実験手法

X-RAY DIFFRACTION (2.6426 Å)