4RUL

Crystal structure of full-length E.Coli topoisomerase I in complex with ssDNA

4RUL の概要

• エントリーDOI: 10.2210/pdb4rul/pdb
• 関連するPDBエントリー: 3PWT 3PX7
• 分子名称: DNA topoisomerase 1, single strand DNA, ZINC ION, ... (6 entities in total)
• 機能のキーワード: topoisomerase 1a, isomerase-dna complex, isomerase/dna
• 由来する生物種: Escherichia coli DH1; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 107370.85

構造登録者

Tan, K.,Chen, B.,Tse-Dinh, Y.C. (登録日: 2014-11-20, 公開日: 2015-11-04, 最終更新日: 2023-09-20)

主引用文献

Tan, K.,Zhou, Q.,Cheng, B.,Zhang, Z.,Joachimiak, A.,Tse-Dinh, Y.C.
Structural basis for suppression of hypernegative DNA supercoiling by E. coli topoisomerase I.
Nucleic Acids Res., 43:11031-11046, 2015

PubMed Abstract: Escherichia coli topoisomerase I has an essential function in preventing hypernegative supercoiling of DNA. A full length structure of E. coli topoisomerase I reported here shows how the C-terminal domains bind single-stranded DNA (ssDNA) to recognize the accumulation of negative supercoils in duplex DNA. These C-terminal domains of E. coli topoisomerase I are known to interact with RNA polymerase, and two flexible linkers within the C-terminal domains may assist in the movement of the ssDNA for the rapid removal of transcription driven negative supercoils. The structure has also unveiled for the first time how the 4-Cys zinc ribbon domain and zinc ribbon-like domain bind ssDNA with primarily π-stacking interactions. This novel structure, in combination with new biochemical data, provides important insights into the mechanism of genome regulation by type IA topoisomerases that is essential for life, as well as the structures of homologous type IA TOP3α and TOP3β from higher eukaryotes that also have multiple 4-Cys zinc ribbon domains required for their physiological functions.

PubMed: 26490962
DOI: 10.1093/nar/gkv1073

実験手法

X-RAY DIFFRACTION (2.9 Å)