4RTQ

Complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with AdoHcy and a 5-bp non-canonical site (GTTTA )

4RTQ の概要

• エントリーDOI: 10.2210/pdb4rtq/pdb
• 関連するPDBエントリー: 2G1P 2ORE 4RTJ 4RTK 4RTL 4RTM 4RTN 4RTO 4RTP 4RTR 4RTS
• 分子名称: DNA adenine methylase, DNA (5'-D(*AP*CP*TP*TP*AP*AP*AP*CP*TP*TP*AP*A)-3'), DNA (5'-D(*TP*TP*TP*AP*AP*GP*TP*TP*TP*AP*AP*G)-3'), ... (6 entities in total)
• 機能のキーワード: dam methylation, gatc recognition, base flipping, bacterial virulence, methylation-independent transcriptional repressor, transferase-dna complex, transferase/dna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 42098.30

構造登録者

Horton, J.R.,Cheng, X. (登録日: 2014-11-15, 公開日: 2015-04-15, 最終更新日: 2023-09-20)

主引用文献

Horton, J.R.,Zhang, X.,Blumenthal, R.M.,Cheng, X.
Structures of Escherichia coli DNA adenine methyltransferase (Dam) in complex with a non-GATC sequence: potential implications for methylation-independent transcriptional repression.
Nucleic Acids Res., 43:4296-4308, 2015

PubMed Abstract: DNA adenine methyltransferase (Dam) is widespread and conserved among the γ-proteobacteria. Methylation of the Ade in GATC sequences regulates diverse bacterial cell functions, including gene expression, mismatch repair and chromosome replication. Dam also controls virulence in many pathogenic Gram-negative bacteria. An unexplained and perplexing observation about Escherichia coli Dam (EcoDam) is that there is no obvious relationship between the genes that are transcriptionally responsive to Dam and the promoter-proximal presence of GATC sequences. Here, we demonstrate that EcoDam interacts with a 5-base pair non-cognate sequence distinct from GATC. The crystal structure of a non-cognate complex allowed us to identify a DNA binding element, GTYTA/TARAC (where Y = C/T and R = A/G). This element immediately flanks GATC sites in some Dam-regulated promoters, including the Pap operon which specifies pyelonephritis-associated pili. In addition, Dam interacts with near-cognate GATC sequences (i.e. 3/4-site ATC and GAT). Taken together, these results imply that Dam, in addition to being responsible for GATC methylation, could also function as a methylation-independent transcriptional repressor.

PubMed: 25845600
DOI: 10.1093/nar/gkv251

実験手法

X-RAY DIFFRACTION (1.997 Å)