4RTE

The X-ray structure of bovine pancreatic ribonuclease incubated in the presence of an excess of cisplatin (1:10 ratio)

4RTE の概要

• エントリーDOI: 10.2210/pdb4rte/pdb
• 関連するPDBエントリー: 4OT4
• 分子名称: Ribonuclease pancreatic, Cisplatin, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: rnase fold, rna cleavage, hydrolase
• 由来する生物種: Bos taurus (bovine,cow,domestic cattle,domestic cow)
• 細胞内の位置: Secreted: P61823
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15014.86

構造登録者

Russo Krauss, I.,Merlino, A. (登録日: 2014-11-14, 公開日: 2015-03-25, 最終更新日: 2024-11-06)

主引用文献

Picone, D.,Donnarumma, F.,Ferraro, G.,Russo Krauss, I.,Fagagnini, A.,Gotte, G.,Merlino, A.
Platinated oligomers of bovine pancreatic ribonuclease: Structure and stability.
J.Inorg.Biochem., 146:37-43, 2015

PubMed Abstract: The reaction between cis-diamminedichloroplatinum(II) (CDDP), cisplatin, a common anticancer drug, and bovine pancreatic ribonuclease (RNase A), induces extensive protein aggregation, leading to the formation of one dimer, one trimer and higher oligomers whose yields depend on cisplatin/protein ratio. Structural and functional properties of the purified platinated species, together with their spontaneous dissociation and thermally induced denaturation, have been characterized. Platinated species preserve a significant, although reduced, ribonuclease activity. The high resistance of the dimers against dissociation and the different thermal unfolding profiles suggest a quaternary structure different from those of the well-known swapped dimers of RNase A.

PubMed: 25756333
DOI: 10.1016/j.jinorgbio.2015.02.011

実験手法

X-RAY DIFFRACTION (1.95 Å)