4RSM

Crystal structure of carbohydrate transporter msmeg_3599 from mycobacterium smegmatis str. mc2 155, target efi-510970, in complex with d-threitol

4RSM の概要

• エントリーDOI: 10.2210/pdb4rsm/pdb
• 分子名称: Periplasmic binding protein/LacI transcriptional regulator, D-Threitol (3 entities in total)
• 機能のキーワード: sugar transporter, abc-type, enzyme function initiative, efi, structural genomics, transport protein, hydrolase
• 由来する生物種: Mycobacterium smegmatis
• 細胞内の位置: Cell membrane ; Lipid-anchor : A0QYB5
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 137874.78

構造登録者

Patskovsky, Y.,Toro, R.,Bhosle, R.,Al Obaidi, N.,Morisco, L.L.,Wasserman, S.R.,Chamala, S.,Attonito, J.D.,Scott Glenn, A.,Chowdhury, S.,Lafleur, J.,Hillerich, B.,Siedel, R.D.,Love, J.,Whalen, K.L.,Gerlt, J.A.,Almo, S.C.,Enzyme Function Initiative (EFI) (登録日: 2014-11-08, 公開日: 2014-12-10, 最終更新日: 2024-02-28)

主引用文献

Huang, H.,Carter, M.S.,Vetting, M.W.,Al-Obaidi, N.,Patskovsky, Y.,Almo, S.C.,Gerlt, J.A.
A General Strategy for the Discovery of Metabolic Pathways: d-Threitol, l-Threitol, and Erythritol Utilization in Mycobacterium smegmatis.
J.Am.Chem.Soc., 137:14570-14573, 2015

PubMed Abstract: We describe a general integrated bioinformatic and experimental strategy to discover the in vitro enzymatic activities and in vivo functions (metabolic pathways) of uncharacterized enzymes discovered in microbial genome projects using the ligand specificities of the solute binding proteins (SBPs) for ABC transporters. Using differential scanning fluorimetry, we determined that the SBP for an ABC transporter encoded by the genome of Mycobacterium smegmatis is stabilized by d-threitol. Using sequence similarity networks and genome neighborhood networks to guide selection of target proteins for pathway enzymes, we applied both in vitro and in vivo experimental approaches to discover novel pathways for catabolism of d-threitol, l-threitol, and erythritol.

PubMed: 26560079
DOI: 10.1021/jacs.5b08968

実験手法

X-RAY DIFFRACTION (1.6 Å)